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Plethysmometer
Plethysmometer
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Highly useful tool in the measurement of small volume changes

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  • IGBMC ILLKIRCH, France
  • SANOFI-AVENTIS PARIS, France
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  • INSERM CLERMONT FERRAND, France
  • BIOPROJET,BIOTECH ST GREGOIRE, FRANCE
  • PORSOLT AND PARTNERS BOULOGNE, FRANCE
  • DUKE UNIVERSITY MEDICAL CENTER Durham, Etats-Unis
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! NEW RESEARCH WORK ! A recent publication by E. Cobos, N. Ghasemlou, D. Araldi, D. Segal, K. Duong et al. in "Pain" highlights the merits of using Bioseb's Plethysmometer: Inflammation-induced decrease in voluntary wheel running in mice: a nonreflexive test for evaluating inflammatory pain and analgesia.

Inflammation-induced decrease in voluntary wheel running in mice: a nonreflexive test for evaluating inflammatory pain and analgesia.
E. Cobos, N. Ghasemlou, D. Araldi, D. Segal, K. Duong et al.
Children's Hospital Boston, F.M. Kirby Neurobiology Center, Boston, USA.
Published in "Pain" (2012-04-30)


Inflammatory pain impacts adversely on the quality of life of patients, often resulting in motor disabilities. Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration of complete Freund's adjuvant (CFA) in mice on a particular form of voluntary locomotion, wheel running, as an index of mobility impairment produced by pain. The distance traveled over 1 hour of free access to activity wheels decreased significantly in response to hind paw inflammation, peaking 24 hours after CFA administration. Recovery of voluntary wheel running by day 3 correlated with the ability to support weight on the inflamed limb. Inflammation-induced mechanical hypersensitivity, measured with von Frey hairs, lasted considerably longer than the impaired voluntary wheel running and is not driving; therefore, the change in voluntary behavior. The CFA-induced decrease in voluntary wheel running was dose-dependently reversed by subcutaneous administration of antiinflammatory and analgesic drugs, including naproxen (10-80 mg/kg), ibuprofen (2.5-20mg/kg), diclofenac (1.25-10mg/kg), celecoxib (2.5-20mg/kg), prednisolone (0.62-5mg/kg), and morphine (0.06-0.5mg/kg), all at much lower doses than reported in most rodent models. Furthermore, the doses that induced recovery in voluntary wheel running did not reduce CFA-induced mechanical allodynia, indicating a greater sensitivity of the former as a surrogate measure of inflammatory pain. We conclude that monitoring changes in voluntary wheel running in mice during peripheral inflammation is a simple, observer-independent objective measure of functional changes produced by inflammation, likely more aligned to the global level of pain than reflexive measures, and much more sensitive to analgesic drug effects.
Presentation

The Digital Water Plethysmometer is designed to provide a highly useful tool in the measurement of small volume changes.
This test is typically used to follow the evolution of the inflammatory response experimentally induced in rodents and to screen potential anti-inflammatory or anti-oedema properties of pharmacological substances.

Operating principle

Basically, the volume transducer is formed by two Perspex tubes interconnected and filled with a conductive solution and a platinum electrode for each chamber. All the system is supported by a stand (included) that can be placed over the control unit.

The water displacement produced by the immersion of the animal paw in the measuring tube is reflected into the second tube, inducing a change in the conductance between the two platinum electrodes.
The Plethysmometer Control Unit detects the conductance changes and generates an output signal to the digital display indicating the volume displacement measured (0.01 ml resolution). The current value remains in the digital display until a new trial starts. The Control Unit is automatically zeroed between successive readings, thus making intermediate adjustments unnecessary.

A remote foot-switch allowing rapid hands-free experiments can be used to set control the end point of the measurement.

The optional SEDACOM software (new version 2.0 available) can be used and represents an easy and convenient way to visualize and export the data on a computer for further analysis.

Parameters Measured

• Mice paw volume (ml)
• Rat paw volume (ml)
Key features

• Computer interface
• Foot-switch control
• Automatic zero adjustment
• New data transfer option with SEDACOM 2.0 software
Volume calibrators
Volume calibrators
Supplied with

• Control Unit with RS232 communication port to PC
• Pedal Switch
• Stimulation Unit
• 1ml or 3ml volume gauge
• SeDaCom Software
• Instructions manual
• Certificate of calibration
• Cables, connectors
• 2 years warranty
• Set of two spare fuses

Options

• LE7000 Thermal printer
Important: 1ml, 3ml or 5ml cell with electrode has to be ordered separately according to your needs



2012
Inflammation-induced decrease in voluntary wheel running in mice: a nonreflexive test for evaluating inflammatory pain and analgesia. (2012)
Inflammation-induced decrease in voluntary wheel running in mice: a nonreflexive test for evaluating inflammatory pain and analgesia.
E. Cobos, N. Ghasemlou, D. Araldi, D. Segal, K. Duong et al.
Children's Hospital Boston, F.M. Kirby Neurobiology Center, Boston, USA.
Published in "Pain" (2012-04-30)


Inflammatory pain impacts adversely on the quality of life of patients, often resulting in motor disabilities. Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration of complete Freund's adjuvant (CFA) in mice on a particular form of voluntary locomotion, wheel running, as an index of mobility impairment produced by pain. The distance traveled over 1 hour of free access to activity wheels decreased significantly in response to hind paw inflammation, peaking 24 hours after CFA administration. Recovery of voluntary wheel running by day 3 correlated with the ability to support weight on the inflamed limb. Inflammation-induced mechanical hypersensitivity, measured with von Frey hairs, lasted considerably longer than the impaired voluntary wheel running and is not driving; therefore, the change in voluntary behavior. The CFA-induced decrease in voluntary wheel running was dose-dependently reversed by subcutaneous administration of antiinflammatory and analgesic drugs, including naproxen (10-80 mg/kg), ibuprofen (2.5-20mg/kg), diclofenac (1.25-10mg/kg), celecoxib (2.5-20mg/kg), prednisolone (0.62-5mg/kg), and morphine (0.06-0.5mg/kg), all at much lower doses than reported in most rodent models. Furthermore, the doses that induced recovery in voluntary wheel running did not reduce CFA-induced mechanical allodynia, indicating a greater sensitivity of the former as a surrogate measure of inflammatory pain. We conclude that monitoring changes in voluntary wheel running in mice during peripheral inflammation is a simple, observer-independent objective measure of functional changes produced by inflammation, likely more aligned to the global level of pain than reflexive measures, and much more sensitive to analgesic drug effects.


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Control Unit Dimensions 280 (W) x 280 (D) x 110 (H)
Stimulation Unit Dimensions 230 (W) x 220 (D) x 300 (H)
Power Supply 220V/110V, 50/60Hz
Starting By panel key or pedal switch
Resolution 3 digits, 0,01 steps
Computer Requirements PC (Windows XP, W7, W8)
Material Composition Clear methacrylate (cell), satinless steel (stand), platinum (electrode)
Maximum number of stations 1 per computer (multiple set-ups also available under request)
Certifications CE Compliant

Model:
LE7500
Plethysmometer
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Accessories :
LE7504
1 ml cell with electrode
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LE7503
3 ml cell with electrode
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LE7505
5 ml cell with electrode
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LE7000
Thermal Printer
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Print version Click to order
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