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ANALGESIA |
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Thermal Place Preference, 2 Temperatures Choice Nociception Test
An operator independant test to study pain thresholds in rodents (mouse and rat) by assessing temperature preference (comfort zone) - a new tool for your analgesia/nociception research
- NIH Bethesda, USA
- UNIVERSITY OF MIAMI Miama, USA
- GRUNENTHAL Aachen, Allemagne
- SANOFI AVENTIS Montpellier, France
- LILLY RESEACH LABORATORIES Indianapolis,IN, USA
- INSTITUT DE GENOMIQUE FONCTIONNELLE Montpellier, France
- NIH Bethesda, USA
- FACULTE DE MEDECINE Clermont Ferrand, France
- UNIVERSITE DE LAUSANNE Lausanne, Suisse
- UNIVERSITE DE PARIS SUD Châtenay Malabry, France
- GRUNENTHAL Aachen, Allemagne
- Swiss Federal Institute of Technolo Lausanne, Suisse
- TEMPLE UNIVERSITY Philadelphia, USA
- MERCK West Point, USA
- KU LEUVEN Leuven, Belgium
- The mechano-activated Kþ channels TRAAK and TREK-1 control both warm and cold perception, by J. Noe, K. Zimmermann, J. Busserolles, E. Deval, A. Alloui,S. Diochot, N. Guy, M. Borsotto,P. Reeh, A. Eschalier and M. Lazdunski, EMBO Journal, 2009
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Presentation
As advised by A. MOQRICH, and published in Moqrich et al (Science 2005, 307: 1468-72), this Thermal Place Preference Test allows researcher to work on unrestrained animals (mice and rats) let free to choose their preferred position (comfort zone) between 2 compartments set at different temperatures. This behavioural assay will allow monitoring temperature preferences, nociceptive thresholds and state in the role of a given gene or a compound on these pain thresholds associated to cold and hot stimulation.
This Temperature Choice Test from Bioseb is investigator-independent, unlike the cold and/or hot plate test: using the traditional plates, an operator can measure the reaction time of an animal (mouse or rat) exposed to a certain temperature. The "two Temperatures Choice Test" will return a nociceptive response without any action from the operator, and the obtained value is a temperature or a temperature range indicating the sensitivity of the animal (mouse or rat) resulting to the exposure to different stimulations (cold or heat).
2 animals (rat or mouse) can be observed simultaneously and independently, making the Thermal Place Preference system remarkably attracting for your analgesia research.
The position of each animal (rodent) can be obtained very easily with the automatic detection software. Results can be transferred directly as an Excel file or as a txt format.
Measured parameters: for each animal, you can retrieve your data as the time spent in each temperature zone, the time of each zone trespassing and the temperature of each zone.
This test is easy to setup :
Using the optional automatic detection software: the operator defines the temperature of each zone. Once the temperatures are stabilized, animals are placed and the acquisition can be launched. The operator doesn't have to be present any more during the nociceptive experiment.
If not using the automatic detection software, temperatures have to be defined manually, and the operator has to measure the time himself.
During the thermal analgesia experience, the operator can visualize the position and movements of each animal on the screen. Raw data acquisition files are stored in a proprietary format to match GLP norms, while a .txt version of the file can be used for further analysis in MS Excel.
Options :
Automatic detection software to measure the position and presence time of the animal (mouse or rat) in each zone, comes with 3 USB cables and a webcam.
!New! Publication:
Bioseb team presents its respectful thanks to research teams of Prof. Lazdunski (Université de Nice-Sophia Antipolis, France) and Prof. Eschalier (Clermont Université, Clermont-Ferrand, France), who used the Two-Temperatures Choice Test during their recent studies:
The mechano-activated Kþ channels TRAAK and TREK-1 control both warm and cold perception, by J. Noe, K. Zimmermann, J. Busserolles, E. Deval, A. Alloui,S. Diochot, N. Guy, M. Borsotto,P. Reeh, A. Eschalier and M. Lazdunski, EMBO Journal, 2009
(Click here to download this article as a PDF file)
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Information published on this Web Site as well as services, product specifications, availability and prices are subject to change without notice. BIOSEB may also make improvements and/or changes in the products and/or the programs described in this Web Site at any time without notice.
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Temperature range |
-3°C to +65°C (room temperature 20 to 25 °C)
For more information about thermal specificities, please check the product page of our Cold+Hot Plate
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Accuracy |
Better than +/- 0.5°C.
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Max Overshoot |
0,5°C
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Electrical power |
150 watts
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Size |
32 x 57 x 45.5 cm (L x w x H including cage)
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Weight |
14 kG
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Animal cage |
330 x 165 x 300 mm, plexiglass
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Time and position measurement |
1s accuracy, video analysis
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Software |
optional, requires Windows XP, and a PC with 512 MO RAM with 3 USB ports |
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Model:
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T2CT
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Thermal Place Preference, 2 Temperatures Choice Nociception Test
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Contact us
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Linked products:
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Bioseb
Tél :+33 (0)820 825 523 Fax :+33 (0)820 825 521
E-Mail : info@bioseb.com
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