! NEW RESEARCH WORK !
A recent publication
by RD Chavez, P Sohn, R Serra in "PLOS One" highlights the merits of using Bioseb's Automatic Foot misplacement apparatus
: Prg4 prevents osteoarthritis induced by dominant-negative interference of TGF-ß signaling in mice
Mouse on the FMA
The Foot Misplacement Apparatus (FMA) is a unique system to objectively detect gait or walking disorders, abnormalities of coordination and motor or reflex problems in small rodents
(rat or mouse) in a highly accurate
way, by recording the number, position and duration of errors (missteps or paws’ slips as well as tail errors) of an animal walking in an opaque corridor on an horizontal ladder towards a dark compartment.
The ladder bars can be removed to increase the difficulty of the exercise or create traps, and two sets of 77 infrared sensors
are placed above and below the ladder to detect the animal’s movement and any paw misplacements, knowing that the number of errors increases with the animals motor deficit and the difficulty of the required task.
The instrument allows you to quantify the recovery of sensorimotor activity
, and is of particular interest for studies on myopathy, ataxia, alcoholism, Parkinson’s and Huntington’s disease, recovery from brain or spinal cord injuries, and much more !
2 compartments (a departure and an arrival box) are located on each side of a long horizontal ladder encased in a corridor. The arrival box is a black compartment that attracts the animal, which walks towards the end of the ladder. On each side of the rungs, infra-red beams and sensors ( 77 sets both above and below the ladder) detect the rodent’s movement and are used to recognize and record the exact position and duration errors in motor control
(missteps or paw slips between any 2 bars).
Furthermore, the Foot Misplacement system is able to differentiate between fore and hind paws
. The space between the rungs can be adjusted to increase the difficulty, and one or more bars can be removed to create traps.
Animals are usually tested across 5 regular runs.
The corridor is removable for an easy and fast cleaning
. Rat and mouse corridors are interchangeable to allow you to test small and large rodents (rats or mice) on the same apparatus
. One corridor for either rat or mouse is included with the instrument - the other version can be ordered separately.
• Fully automatic, operator-independent
• Aversive starting box with cold white blinking light
• Interchangeable rat/mouse corridors on the same instrument
• Two sets of 77 infrared sensors provide high accuracy, and great reliability
• Self-powered by USB port from PC
Domains of application|
• Functional, toxicological, and genetic models
• Studies on toxicosis, alcoholism
• Resarch on myopathy and ataxia
• Research on Parkinson's and Huntington’s disease
• Recovery from brain or spinal cord injuries
Bioseb’s Foot Misplacement Apparatus software records data collected by the sensors and determines parameters for all errors from paws (both front and hind) and tail.
It generates a distinct results file for each animal, which is easily exportable into Excel format. The software also filters sensors information, allows adjustment of the misplacement duration detection, automatically sorts out errors from front legs, back legs and tail and directly calculates the time/report ratio of the course and error rate.
Following recorded parameters are included into the detailed experimental report :
• Total crossing time
• Input and output latency time
• Number of errors (missteps or slips)
• Type of errors (front paws, hind paws or tail)
• Duration of each error
• Position of each error
Screenshots from the foot misplacement system software