! NEW RESEARCH WORK !
A recent publication
by S Cornet, C P in "Toxicon: X" highlights the merits of using Bioseb's Dynamic Weight Bearing 2.0
: The use of the dynamic weight bearing test to assess the effects of acute, intramuscularly administered botulinum neurotoxin type A1 in rats
2006 - Incapacitance Test / Static Weight Bearing (SWB) :
The first instrument measuring spontaneous pain for your research on nociception and analgesia. Our system allows you to measure the weight distribution of a rodent comfortably maintained on its hind paws in a dedicated holder.
2008 - Basic Dynamic Weight Bearing test (BDWB):
This new instrument allows you to measure the weight distribution on all four paws without a restrainer.
2011 - Advanced Dynamic Weight Bearing test (ADWB):
A new software version of the DWB allowing video tracking of the animal for running the test semi-automatically
2018 - Dynamic Weight Bearing test 2.0 (DWB2):
New 3-tab software with improved automation behavior and major, new, innovative functionalities.
Advanced Dynamic Weight Bearing test
Detail with a mouse
Ethical considerations as well as the necessity to study comfort and evoked pain levels on rodents lead to the development of tests and procedures allowing researchers to conduct pain assessment without having to apply a stimulus on the animals. These tests, called "Incapacitance" tests, measure the weight distribution on both hind paws of rodents maintained in a restrainer. Restraining, however, induced higher level of stress for the test subjects.
Since its launching in 2008, Bioseb's Dynamic Weight Bearing test is the only rodent evoked pain test not requiring animal restraint
. It is also the only instrument allowing you to precisely measure changes in Postural Equilibrium
in rodents by assessing their weight distribution on each one of their 4 paws.
Bioseb's DBW thus allows you to work with freely moving animals
in a transparent cage adapted to the animal being tested (rats or mice). This unique characteristic significantly reduces restrainer-induced stress levels and therefore greatly increases the reliability of the results.
This instrument, after having been validated in over thirty publications
, has enabled researchers to answer numerous interrogations pertaining to non-evoked pain models, as well as neuropathic, inflammatory or osteoarthritic conditions. More recently, the application framework of our DWB (Dynamic Weight Bearing) has been extended: the instrument is not only a must-have for pain models, but its postural analysis ability has proven to be an ideal tool for studies on skeletal diseases (fractures, cancers, arthritis...), CNS (Central Nervous System) pathologies, as well as diseases connected to the vestibular system (vertigo).
The animal is placed into the DWB (Dynamic Weight Bearing) enclosure for a duration of approximately 5 minutes.
During the test, the DWB relies on two different technologies :
A matrix comprising around 2000 high precision force sensors
(0.02 g for mice and 0.15 g for rats): this matrix is embedded in the floor of the enclosure where the animal is free to move as it pleases. The force sensors measure the weight distribution on each of the four paws of the animal in grams. The instrument uses different sensors for rats or mice in order to provide a suitable sensitivity level (0-4g for mice and 0-40g for rats).
: the animal is filmed from above using a high definition camera. The video feed is analyzed in real-time during the test thanks to our tracking software allowing a precise analysis of the animal's posture.
Our software can accurately analyze the animal's position during the entire duration of the test in order to automatically identify the location of each of the four paws on the force sensors.
Data acquisition from the force sensors and the camera happens at a high frequency
(30 Hz): the DWB is able to analyze rapid position changes, thus providing complete measurements allowing a precise tracking of postural evolutions in moving or still rodents.
New DWB2 Dynamic Weight Bearing Test
The new DWB2 version offers unprecedented improvements increasing both speed and software user experience while optimizing results accuracy and reliability.
Software screenshot Analysis page
New DWB2 Dynamic Weight Bearing Test
Software Results page
The DWB2 software sports a new 3-tab interface
. With its faster and more intuitive interface, it will be a breeze to use for all the operators in your lab.
The sensors sampling rate has been increased
to 30 measurements per second
. Results are more comprehensive and accurate, thus allowing you to determine all postural modifications of both moving and still animals.
We re-designed the automatic postural analysis algorithms
to increase their precision. These algorithms are now supported by a complete database of pre-recorded postures recorded in both rats and mice. Data acquisition and analysis are greatly accelerated and more accurate.
A Trust Index
has been implemented for automatic results analysis. This saves a lot of time for the operator who can then choose to either only retain data with a high trust index, or to proceed to a more in-depth manual analysis for lower trust indexes.
A group management module
is now embedded in the software, as well as animal lists in order to follow GLP procedures and avoid errors, thus increasing results' reliability.
Furthermore, new improvements will soon be released. We are currently working on new computation parameters
such as the support polygon
(encompassing several data points pertaining to the distance between the paws and the position of the gravity center) as well as the BOS
(Base or Support, or distance between both hind paws).
Instrument versions and installation
Weight for each paw (g and % total animal weight)
Weight for grouped front and rear paws (g and %total animal weight)
Left/Right and Front/Rear weight ratio
Surface for each paw (mm²)
Surface for grouped front and rear paws (mm²)
Variability (standart deviation/mean) for each parameter
Parameters are given for each posture and as a mean for the whole experiment
Duration of different postures (4 paws, rearing
) over the whole experiment (s)
Total time spent on each paw over the whole experiment (s)
Advantages of our new Biosebs dynamic weight bearing system over static incapacitance systems
Analyzes all four paws
Convenient for manipulating large numbers of rodents
Unnecessary stress associated with restraint is eliminated
The new version of our Dynamic Weight Bearing Apparatus offers ground-breaking new functionalities:
Experiment management: define experiment parameters, import a list of subjects and run the total experience. View and analyze subjects on an individual or group basis.
Possibility to run longer experiments
Record up to 30 measurements per second (better accuracy on animal positioning)
Perform detailed analysis on immobility, mobility, or both conditions
Improved scoring algorithm detecting 80% of the paws
Special tool to validate video parameters
Improved tools for scoring, undo/redo option, fusion , etc
Coming soon: special model for guinea pigs
Coming soon: new computation modules - support polygon & base of support
Applications for your research|
Pain - Nociception/Analgesia
Bone cancer models
Post surgery models
Pelvic pain models
Cerebrovascular accident models
Spinal cord injuries recovery process
Other research applications
Calcaneal tendon disease
Crucial ligaments disease
All DWB1 users are eligible for an upgrade to the new DWB2 version. Your hardware is 100% compatible - a simple software upgrade will be required.
Acquisitions from the DWB1 can be analyzed using the DWB2 software in order to benefit from the new interface and powerful analysis modules.
The DWB is available in 3 different versions: RAT (BIO-DWB-AUTO-R), MICE (BIO-DWB-AUTO-M), RAT-MICE COMBO (BIO-DWB-AUTO-DU).
New Advanced Weight Bearing System for Mouse and Rat, by Bioseb: camera, cover, cage and animal