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PHECOMP PLATFORM
(Model: Multitake - For mice. Home cage included)
Innovative and appropriate equipment for measuring food/liquid consumption and correlated motor activity in rats and mice

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! NEW RESEARCH WORK ! A recent publication by G Le DrŽan, AL Pocheron, H Billard, I Grit, A Pagniez, et al in "Nutrients" highlights the merits of using Bioseb's PheComp Platform: Neonatal Consumption of Oligosaccharides Greatly Increases L-Cell Density without Significant Consequence for Adult Eating Behavior

Neonatal Consumption of Oligosaccharides Greatly Increases L-Cell Density without Significant Consequence for Adult Eating Behavior
G Le DrŽan, AL Pocheron, H Billard, I Grit, A Pagniez, et al
Nantes Universite, INRA, UMR1280, PhAN, F-44000 Nantes, France
Published in "Nutrients" (2019-08-21)


Oligosaccharides (OS) are commonly added to infant formulas, however, their physiological impact, particularly on adult health programming, is poorly described. In adult animals, OS modify microbiota and stimulate colonic fermentation and enteroendocrine cell (EEC) activity. Since neonatal changes in microbiota and/or EEC density could be long-lasting and EEC-derived peptides do regulate short-term food intake, we hypothesized that neonatal OS consumption could modulate early EECs, with possible consequences for adult eating behavior. Suckling rats were supplemented with fructo-oligosaccharides (FOS), beta-galacto-oligosaccharides/inulin (GOS/In) mix, alpha-galactooligosaccharides (alphaGOS) at 3.2 g/kg, or a control solution (CTL) between postnatal day (PND) 5 and 14/15. Pups were either sacrificed at PND14/15 or weaned at PND21 onto standard chow. The effects on both microbiota and EEC were characterized at PND14/15, and eating behavior at adulthood. Very early OS supplementation drastically impacted the intestinal environment, endocrine lineage proliferation/differentiation particularly in the ileum, and the density of GLP-1 cells and production of satiety-related peptides (GLP-1 and PYY) in the neonatal period. However, it failed to induce any significant lasting changes on intestinal microbiota, enteropeptide secretion or eating behavior later in life. Overall, the results did not demonstrate any OS programming effect on satiety peptides secreted by L-cells or on food consumption, an observation which is a reassuring outlook from a human perspective.

Presentation

The PHECOMP system is an innovative and appropriate equipment for measuring food/liquid consumption and correlated motor activity in studies designed to assess compulsive behaviours in mice.

Operating principle

The BIOSEB PHECOMP system uses the weight transducer technology for measuring food and drink consumption, thus allowing a continuous signal and then a precise analysis of the rodent meal pattern by the software. The mouse home cages can be associated to up to 4 external units for food or drink (user-defined). The system registers absorbed food and its wastage by means of weight transducers of very high stability mounted into the platform supporting each cage. The quantity of water drunk from a nipple, and any possible dripping, are also accounted using the same system of transducers. Rodent activity and rearing are recorded simultaneously by the means of 2 dimensional IR frames.

The signals from each weight transducer unit and the IR frame are amplified, digitalized and send to the BIOSEB COMPULSE software for storing and further analysis. A track is also recorded and can be analyzed by the BIOSEB Actitrack software for further analysis of animal position. Only one cable connects the platforms between them and the last one is linked to the PC using RS232/USB port.

Domains of application

The modular design of the PHECOMP system allows a wide range of experimental procedures for characterizing the evolution of the rodent compulsive behaviour along the experimental process:
• in general schedule for behavioural testing obese versus non-obese mice,
• in a set of experimental interventions suitable for the detection of causal factors including compulsive components (choice test, bitter test, starvation...),
• for screening anti-obesity compound developed by the pharmaceutical companies.

Parameters Measured

• Food or drink consumption by user-defined interval of time (COMPULSE)
• Meal pattern analysis (COMPULSE)
• Spontaneous activity by user-defined interval of time (ACTITRACK)
• Ambulatory behavior by user-defined interval of time (ACTITRACK)
• Stereotyped behaviors by user-defined interval of time (ACTITRACK)
• Rearings (ACTITRACK)
• Movement pattern: resting/slow/fast movements... (ACTITRACK)
• Permanence time and entries into user-defined zones (ACTITRACK)

Key features

• Multiple combinations of dispensers
• Uninterrupted recording
• Use very high stability weight transducers
• Registers food and liquid wastage
• External dispensers avoid any influence on the living space of the mice as well as unwanted artifacts


Publications (Click on an article to show details and read the abstract)

METABOLISM
- Food intake / Nutrition -
Neonatal Consumption of Oligosaccharides Greatly Increases L-Cell Density without Significant Consequence for Adult Eating Behavior (2019)
Neonatal Consumption of Oligosaccharides Greatly Increases L-Cell Density without Significant Consequence for Adult Eating Behavior
G Le DrŽan, AL Pocheron, H Billard, I Grit, A Pagniez, et al
Nantes Universite, INRA, UMR1280, PhAN, F-44000 Nantes, France
Published in "Nutrients" (2019-08-21)

Oligosaccharides (OS) are commonly added to infant formulas, however, their physiological impact, particularly on adult health programming, is poorly described. In adult animals, OS modify microbiota and stimulate colonic fermentation and enteroendocrine cell (EEC) activity. Since neonatal changes in microbiota and/or EEC density could be long-lasting and EEC-derived peptides do regulate short-term food intake, we hypothesized that neonatal OS consumption could modulate early EECs, with possible consequences for adult eating behavior. Suckling rats were supplemented with fructo-oligosaccharides (FOS), beta-galacto-oligosaccharides/inulin (GOS/In) mix, alpha-galactooligosaccharides (alphaGOS) at 3.2 g/kg, or a control solution (CTL) between postnatal day (PND) 5 and 14/15. Pups were either sacrificed at PND14/15 or weaned at PND21 onto standard chow. The effects on both microbiota and EEC were characterized at PND14/15, and eating behavior at adulthood. Very early OS supplementation drastically impacted the intestinal environment, endocrine lineage proliferation/differentiation particularly in the ileum, and the density of GLP-1 cells and production of satiety-related peptides (GLP-1 and PYY) in the neonatal period. However, it failed to induce any significant lasting changes on intestinal microbiota, enteropeptide secretion or eating behavior later in life. Overall, the results did not demonstrate any OS programming effect on satiety peptides secreted by L-cells or on food consumption, an observation which is a reassuring outlook from a human perspective.


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Accuracy <0.03 mg for both food and drink
Mouse Home cage dimensions 189 x 297 x 128 (H) mm
Platform dimensions 410 x 320 x 75 (H) mm
LE8827 Activity IR frame 400 x 290 x 13 (H) mm, 16x16 beams (16 mm spaced)
LE8827 Rearing IR frame 400 x 16 (H) mm, 16 beams (16 mm spaced)

Model:
Multitake
PheComp Platform
For mice. Home cage included Contact us

Related products:
ACTITRACK
Acquisition software: ACTITRACK
Contact us
COMPULSE
COMPULSE software
Contact us
MULTITAKE-RAT
PheComp Platform
For rats. Home cage included Contact us
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