! NEW RESEARCH WORK !
A recent publication
by J. Descoeur, V. Pereira, A. Pizzoccaro, A. Francois, B. Ling et al. (Team of Dr Bourinet) in "EMBO Molecular Medicine" highlights the merits of using Bioseb's Jump Test
: Oxaliplatin-induced cold hypersensitivity is due to remodelling of ion channel expression in nociceptors.
Following the publications of I. Yalcin et al. (The Journal of Pain, 2009) and complementary works from team of Dr Bourinet (EMBO J.Descoeur et al, 2011) regarding hypersensitivity to cold stimulus, BIOSEB has developed an entirely new set-up for the evaluation of the escaping behaviour of mice experimenting a ramp of temperature (cold and hot) over an aluminium plate
The entire instrument is based on our popular Hot and cold Plate hardware
, built around a metal plate which can be heated to 65°C and cooled to -3°C (with an ambient temperature between 20°C and 25°C), which already opened new investigation fields for your analgesia and nociception research. The classical setting is completed with a special back lighting plate and a HD camera sensitive to the back light wavelength placed in front of the Hot and Cold Plate.
A dedicated software controls the whole test and allows you to:
• Program and control the ramp of temperature
• Collect/record/compress the video
• Detect the jumps of the mouse within its cage and in total darkness
• Display graphic of jump over time and jumps over range of temperature
The test has been mostly used to measure pharmacological effects of cancer treatment to cold sensitivity on mice
A ramp is generally programmed from 30°C to 1 °C and the distribution of jump recorded over the whole experiment. Metrology wise, the Jump Test is accurate to less than 0,5°C (EEC metrology standard) and perfectly constant in the animal holder system.
The JUMP TEST is an automatic analgesia apparatus
which frees time to the experimenter to perform other works on bench. It secures the counting, and stores video as a backup if additional evaluation is needed later.
JTET Software for the Jump Test
• Fully automatic
• Works in total darkness
• Owner of HCP
eligible for update
• Evolutive hardware (T2CT Gradient Test
• Principle validated and published
• Jumps counting
• Distribution of jumps/time
• Distribution of jumps vs/°C
Domains of application
• Differentiate thermal allodynia from hyperalgesia models
• Study peripheral pain sensitization for mice in a single paradigm
• Measure pharmacological effects of cancer treatment on mice's sensitivity to cold
• Measure efficacy of analgesics
• Studies on thermal nociception in mouse models: allodynia, hyperalgesia of inflammatory pain models, capsaicin-induced inflammation, effects of carrageenins
• Chemotherapy-induced neuropathy: application on animals exposed to oxalyplatin and developing hypersensitivity to cold
• Studies on morphine and naloxone dependence
• Hot cold plate and its cage
• Lighting and special camera
• JTET software, cables and instruction manual