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SPONTANEOUS ACTIVITY WHEELS
(Model: BIO-ACTIVW-R - For Rats)
Spontaneous activity wheel for rodent is an easy way to quantify rodents’ spontaneous activity in their home cage environment - particularly well suited for the mouse. The embedded electronics perform a wide range of measurements, including: wheel turns, average/min/max speed, acceleration, total time in the wheel, number of accesses to the wheel, time on the wheel, etc. A useful tool for studies on Drug screening, Phenotyping and Neuromuscular diseases. Now with a software-independent LCD display, and a brand-new software controlling up to 64 wheels!

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  • SWISS FEDERAL INSTITUTE OF TECHNOLOGY Lausanne, Suisse
  • INSERM Paris, France
  • IPSEN Les Ulis, France
  • UNIVERSITY OF GENEVA Geneve, Suisse
  • CNRS Angers, France
  • CHILDRENS HOSPITAL Boston, France
  • PHARMACOLOGY GRANADA UNIVERSITY GRANADA, SPAIN
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! NEW RESEARCH WORK ! A recent publication by S Ni, Z Ling, X Wang, Y Cao, T Wu, R Deng, JL Crane et al in "Nature Communications" highlights the merits of using Bioseb's Spontaneous activity wheels: Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice

Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice
S Ni, Z Ling, X Wang, Y Cao, T Wu, R Deng, JL Crane et al
Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
Published in "Nature Communications" (2019-12-10)


Spinal pain is a major clinical problem, however, its origins and underlying mechanisms remain unclear. Here we report that in mice, osteoclasts induce sensory innervation in the porous endplates which contributes to spinal hypersensitivity in mice. Sensory innervation of the porous areas of sclerotic endplates in mice was confirmed. Lumbar spine instability (LSI), or aging, induces spinal hypersensitivity in mice. In these conditions, we show that there are elevated levels of PGE2 which activate sensory nerves, leading to sodium influx through Nav 1.8 channels. We show that knockout of PGE2 receptor 4 in sensory nerves significantly reduces spinal hypersensitivity. Inhibition of osteoclast formation by knockout Rankl in the osteocytes significantly inhibits LSI-induced porosity of endplates, sensory innervation, and spinal hypersensitivity. Knockout of Netrin-1 in osteoclasts abrogates sensory innervation into porous endplates and spinal hypersensitivity. These findings suggest that osteoclast-initiated porosity of endplates and sensory innervation are potential therapeutic targets for spinal pain.
Activity wheel for rodent, by Bioseb
Activity wheel for rat, by Bioseb
Presentation

Our new Spontaneous Activity Wheel is an easy and cost-efficient way to quantify rodents' spontaneous activity in their home cage environment (type IIL and III cages), and can be used for both mice and rats (two different models available).

The system allows you to record the parameters pertaining to the voluntary exercise of the animal, which can freely decide upon its timing and intensity. Monitoring and recording typically takes place over extended periods of time (several days or weeks) in order to analyze the differences in behavior and exercising patterns induced by drugs or surgical manipulation. Though the analysis focuses on spontaneous activity, it is highly relevant for long-term studies on alterations of the circadian rhythm.

Measurements include the distance run both ways, the number of wheel turns, average/min/max speed, acceleration, total time in the wheel, and can be displayed in statistical form or sorted by time periods. Periods can be customized for facilitating further analysis.

An ideal tool for studies on Drug screening, Phenotyping and Neuromuscular diseases.

Operating principle

New embedded LCD screen of Bioseb's Spontaneous activity wheel for rodent
New embedded LCD screen:
Automatic counter
Highly reliable sensors offering an excellent angular resolution (22°) as well as time resolution (0,1 seconds for up to 64 cages) accurately records the animal's spontaneous exercising behavior, even allowing the study of the rodent’s acceleration and power pattern.

A clever design includes a coding disk as well as an LCD screen counter and Infrared sensors, as well as a “standard” cage construction from stainless steel and perpex ensures that the system is both durable and easy to use and maintain in the daily laboratory operation.

A complete digital solution based on Bioseb’s ACTIVW-SOFT software allows you to monitor up to 64 cages simultaneously via an external connection to the PC USB port (up to 5 m between cage and PC).

Key features

• Allow monitoring of spontaneous activity
• High angle and time resolution allows recording of acceleration pattern
• Practical and sturdy design is adapted for durable use and easy cleaning
• Software allows monitoring of up to 64 wheels simultaneously
• Cost-effective setting not requiring the presence of an operator (unlike treadmills)
• Now with an LCD display!
• Works for both rats and mice
Domains of application

• Drug screening
• Phenotyping
• Neuromuscular diseases
• Parkinson disease
• Duchenne muscular dystrophy

Dedicated software ACTIVW-SOFT

Our ACTIVW-SOFT software offers a complete solution to manage rodents groups (mice or rats), allowing you to import/export and edit groups, as well as setup delayed acquisition start, and pause recording (for instance during cage cleaning). Data is recorded at 15Hz, then integrated at 1Hz, and can be re-compiled in analytical mode on an adjustable period of 1 sec up to 999 minutes.

Bioseb's dedicated ACTIVW-SOFT lets you visualize data in a convenient control panel, for each wheel, or for a group of wheels.

Bioseb's dedicated software for rodents' activity wheels allows customized settings for the data acquisition averaging periods (from 1second), the duration of recording, the latency period prior to account for Wheel movement, etc. Those settings can be defined according the user's needs.

Results include:
• A data file computed per period with informations of speed, acceleration, wheel rotations...
• Total time of the animal in the wheel with break down in number of rotating occurences (using the preset latency time)
• Outputs: via txt file in Microsoft Excel format.


Publications (Click on an article to show details and read the abstract)

PAIN
- General pain -
Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice (2019)
Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice
S Ni, Z Ling, X Wang, Y Cao, T Wu, R Deng, JL Crane et al
Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
Published in "Nature Communications" (2019-12-10)

Spinal pain is a major clinical problem, however, its origins and underlying mechanisms remain unclear. Here we report that in mice, osteoclasts induce sensory innervation in the porous endplates which contributes to spinal hypersensitivity in mice. Sensory innervation of the porous areas of sclerotic endplates in mice was confirmed. Lumbar spine instability (LSI), or aging, induces spinal hypersensitivity in mice. In these conditions, we show that there are elevated levels of PGE2 which activate sensory nerves, leading to sodium influx through Nav 1.8 channels. We show that knockout of PGE2 receptor 4 in sensory nerves significantly reduces spinal hypersensitivity. Inhibition of osteoclast formation by knockout Rankl in the osteocytes significantly inhibits LSI-induced porosity of endplates, sensory innervation, and spinal hypersensitivity. Knockout of Netrin-1 in osteoclasts abrogates sensory innervation into porous endplates and spinal hypersensitivity. These findings suggest that osteoclast-initiated porosity of endplates and sensory innervation are potential therapeutic targets for spinal pain.

- Inflammatory pain -
Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats. (2017)
Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats.
Favero G, Trapletti V, Bonomini F, Stacchiotti A, Lavazza A, Rodella LF, Rezzani R
University of Brescia, Brescia, Italy
Published in "International Journal of Molecular Sciences" (2017-06-29)

Fibromyalgia is a chronic syndrome characterized by widespread musculoskeletal pain and an extensive array of other symptoms including disordered sleep, fatigue, depression and anxiety. Important factors involved in the pathogenic process of fibromyalgia are inflammation and oxidative stress, suggesting that ant-inflammatory and/or antioxidant supplementation might be effective in the management and modulation of this syndrome. Recent evidence suggests that melatonin may be suitable for this purpose due to its well known ant-inflammatory, antioxidant and analgesic effects. Thus, in the current study, the effects of the oral supplementation of melatonin against fibromyalgia-related skeletal muscle alterations were evaluated. In detail, 90 Sprague Dawley rats were randomly treated with reserpine, to reproduce the pathogenic process of fibromyalgia and thereafter they received melatonin. The animals treated with reserpine showed moderate alterations at hind limb skeletal muscles level and had difficulty in moving, together with significant morphological and ultrastructural alterations and expression of inflammatory and oxidative stress markers in the gastrocnemius muscle. Interestingly, melatonin, dose and/or time dependently, reduced the difficulties in spontaneous motor activity and the musculoskeletal morphostructural, inflammatory, and oxidative stress alterations. This study suggests that melatonin in vivo may be an effective tool in the management of fibromyalgia-related musculoskeletal morphofunctional damage.

Inflammation-induced decrease in voluntary wheel running in mice: a nonreflexive test for evaluating inflammatory pain and analgesia. (2012)
Inflammation-induced decrease in voluntary wheel running in mice: a nonreflexive test for evaluating inflammatory pain and analgesia.
E. Cobos, N. Ghasemlou, D. Araldi, D. Segal, K. Duong et al.
Children's Hospital Boston, F.M. Kirby Neurobiology Center, Boston, USA.
Published in "Pain" (2012-04-30)

Inflammatory pain impacts adversely on the quality of life of patients, often resulting in motor disabilities. Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration of complete Freund's adjuvant (CFA) in mice on a particular form of voluntary locomotion, wheel running, as an index of mobility impairment produced by pain. The distance traveled over 1 hour of free access to activity wheels decreased significantly in response to hind paw inflammation, peaking 24 hours after CFA administration. Recovery of voluntary wheel running by day 3 correlated with the ability to support weight on the inflamed limb. Inflammation-induced mechanical hypersensitivity, measured with von Frey hairs, lasted considerably longer than the impaired voluntary wheel running and is not driving; therefore, the change in voluntary behavior. The CFA-induced decrease in voluntary wheel running was dose-dependently reversed by subcutaneous administration of antiinflammatory and analgesic drugs, including naproxen (10-80 mg/kg), ibuprofen (2.5-20mg/kg), diclofenac (1.25-10mg/kg), celecoxib (2.5-20mg/kg), prednisolone (0.62-5mg/kg), and morphine (0.06-0.5mg/kg), all at much lower doses than reported in most rodent models. Furthermore, the doses that induced recovery in voluntary wheel running did not reduce CFA-induced mechanical allodynia, indicating a greater sensitivity of the former as a surrogate measure of inflammatory pain. We conclude that monitoring changes in voluntary wheel running in mice during peripheral inflammation is a simple, observer-independent objective measure of functional changes produced by inflammation, likely more aligned to the global level of pain than reflexive measures, and much more sensitive to analgesic drug effects.

- Neuropathic pain -
Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy (2018)
Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy
LA Gri_ths, NA Duggett, AL Pitcher, SJL Flatter
Wolfson Centre for Age-Related Diseases, King’s College London, London, UK
Published in "Pain Research and Management" (2018-06-03)

Paclitaxel-inducedneuropathicpainisamajordose-limitingsidee_ectofpaclitaxeltherapy.Thisstudycharacterisesavarietyof rat behavioural responses induced by intermittent administration of clinically formulated paclitaxel. 2mg/kg paclitaxel or equivalentvehiclewasadministeredintraperitoneallyondays0,2,4,and6toadultmaleSprague-Dawleyrats.Evokedpain-like behaviours were assessed with von Frey _laments, acetone, or radiant heat application to plantar hind paws to ascertain mechanical, cold, or heat sensitivity, respectively. Motor coordination was evaluated using an accelerating RotaRod apparatus. Ongoing pain-like behaviour was assessed via spontaneous burrowing and nocturnal wheel running. Mechanical and cold hypersensitivity developed after a delayed onset, peaked approximately on day 28, and persisted for several months. Heat sensitivity and motor coordination were unaltered in paclitaxel-treated rats. Spontaneous burrowing behaviour and nocturnal wheelrunningweresigni_cantlyimpairedonday28,butnotonday7,indicatingongoingpain-likebehaviour,ratherthanacute drug toxicity. This study comprehensively characterises a rat model of paclitaxel-induced peripheral neuropathy, providing the _rstevidenceforongoingpain-likebehaviour,whichoccursinparallelwithmaximalmechanical/coldhypersensitivity.Wehope that this new data improve the face validity of rat models to better re_ect patient-reported pain symptoms, aiding translation of new treatments to the clinic.

Peripherally Acting _-Opioid Receptor Agonists Attenuate Ongoing Pain-associated Behavior and Spontaneous Neuronal Activity after Nerve Injury in Rats (2018)
Peripherally Acting _-Opioid Receptor Agonists Attenuate Ongoing Pain-associated Behavior and Spontaneous Neuronal Activity after Nerve Injury in Rats
V Tiwari, M Anderson, F Yang, V Tiwari, Q Zheng, et al.
Division of Pain Medicine, Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland, USA
Published in "Anesthesiology" (2018-03-29)

Methods: Using conditioned place preference assay, the authors tested whether animals show a preference to the environment associated with drug treatment. Wide-dynamic range and dorsal root ganglion neuronal activities were measured by electrophysiology recording and calcium imaging.
Results: Nerve-injured animals stayed longer in dermorphin [D-Arg2, Lys4] (1Ð4) amideÐpaired chamber after conditioning than during preconditioning (rats: 402.4 ± 61.3 vs. 322.1 ± 45.0 s, 10 mg/kg, n = 9, P = 0.009; mice: 437.8 ± 59.4 vs. 351.3 ± 95.9 s, 2 mg/kg, n = 8, P = 0.047). Topical ganglionic application of dermorphin [D-Arg2, Lys4] (1Ð4) amide (5 _M, 1 _l, n = 5) reduced the numbers of small-diameter dorsal root ganglion neurons that showed spontaneous activity (1.1 ± 0.4 vs. 1.5 ± 0.3, P = 0.044) and that were activated by test stimulation (15.5 ± 5.5 vs. 28.2 ± 8.2, P = 0.009) after injury. In neuropathic rats, dermorphin [D-Arg2, Lys4] (1Ð4) amide (10 mg/kg, n = 8) decreased spontaneous firing rates in wide-dynamic range neurons to 53.2 ± 46.6% of predrug level, and methylnaltrexone (5 mg/kg, n = 9) blocked dermorphin [D-Arg2, Lys4] (1Ð4) amideÐinduced place preference and inhibition of wide-dynamic range neurons. Dermorphin [D-Arg2, Lys4] (1Ð4) amide increased paw withdrawal threshold (17.5 ± 2.2 g) from baseline (3.5 ± 0.7 g, 10 mg/kg, n = 8, P = 0.002) in nerve-injured rats, but the effect diminished after repeated administrations.
Conclusions: Peripherally acting _-opioids may attenuate ongoing pain-related behavior and its neurophysiologic correlates. Yet, repeated administrations cause antiallodynic tolerance.

Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats. (2017)
Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats.
Favero G, Trapletti V, Bonomini F, Stacchiotti A, Lavazza A, Rodella LF, Rezzani R
University of Brescia, Brescia, Italy
Published in "International Journal of Molecular Sciences" (2017-06-29)

Fibromyalgia is a chronic syndrome characterized by widespread musculoskeletal pain and an extensive array of other symptoms including disordered sleep, fatigue, depression and anxiety. Important factors involved in the pathogenic process of fibromyalgia are inflammation and oxidative stress, suggesting that ant-inflammatory and/or antioxidant supplementation might be effective in the management and modulation of this syndrome. Recent evidence suggests that melatonin may be suitable for this purpose due to its well known ant-inflammatory, antioxidant and analgesic effects. Thus, in the current study, the effects of the oral supplementation of melatonin against fibromyalgia-related skeletal muscle alterations were evaluated. In detail, 90 Sprague Dawley rats were randomly treated with reserpine, to reproduce the pathogenic process of fibromyalgia and thereafter they received melatonin. The animals treated with reserpine showed moderate alterations at hind limb skeletal muscles level and had difficulty in moving, together with significant morphological and ultrastructural alterations and expression of inflammatory and oxidative stress markers in the gastrocnemius muscle. Interestingly, melatonin, dose and/or time dependently, reduced the difficulties in spontaneous motor activity and the musculoskeletal morphostructural, inflammatory, and oxidative stress alterations. This study suggests that melatonin in vivo may be an effective tool in the management of fibromyalgia-related musculoskeletal morphofunctional damage.



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Standard rat cage Type III Polycarbonate
Wheel and cover Stainless steel
Local display LCD Screen: CW/CCW/Total/Rat name
Overall dimensions L x W x H = 48 x 31,5 x 47 cm
Wheel dimensions Diameter : 34 cm, Lane width : 7cm
Sampling rate 15 Hz
Angular resolution 22° in both directions
Minimum period 1 sec
Power supply 240 / 110 V, 50 / 60 Hz
Output requires 2 USB Ports
Software W7 / W8 64 bits

Model:
BIO-ACTIVW-R
Spontaneous activity wheels
For Rats Contact us

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