! NEW RESEARCH WORK !
A recent publication
by D Romeo-Guitart, C Casas in "Cells" highlights the merits of using Bioseb's Electronic Von Frey 5 with embedded camera
: NeuroHeal Treatment Alleviates Neuropathic Pain and Enhances Sensory Axon Regeneration
New EVF Software with embedded video
16 years after the creation of our first Electronic Von Frey instrument, Bioseb is revolutionizing this standard test for determining the mechanical sensitivity threshold in rodents (rats and mice)
A well-known issue with classical Electronic Von Frey instruments is the necessity for the operator to bend into an uncomfortable posture in order to stimulate under the rodents' paws. Despite using more ergonomic workplace configurations (lower seats and elevated desks), the operation can remain strenuous or even painful for the operator when repeated over large cohorts of animals.
In order to solve this issue, we embedded a camera inside the stimulator handle, which allows you to record and display video directly from underneath the paws. In conjunction with our dedicated software, this innovation allowed us to re-think the position of the operator during the test.
When used on a tablet or touch-screen, the EVF5 software allows operators to visualize the video stream in real time without having to bend over. Furthermore, it integrates all additional functionalities that might be required during such a test:
Animal lists management.
Real-time curves display: visualizing the force over time helps operators improve their execution and the repeatability of their measurement
A table allowing you to assign each measurement to the corresponding paw by directly clicking on the touch screen.
A replay function for both videos and force curves allowing the operators to delete invalid measurements.
Exporting results to an Excel spreadsheet.
Built around a stimulator handle connected to a touchscreen tablet with an embedded, dedicated software, the instrument was designed to offer operators a complete working environment for the Von Frey Test which does not require them to bend over during stimulation.
The operator creates a new experiment on the dedicated software and configures their animal list as well as the different paws that they wish to stimulate (usually the hind paws). The test is then started by taking the stimulator handle and observing the target zones under the animal's paw directly via the video stream on the touchscreen tablet, without having to bend over to look under the plantar surface.
Stimulation is usually applied on the rodent's hind paws. The paw's withdrawal following the mechanical stimulation is recorded as a response to the stimulus. During measurement, the operator can observe in real-time and thus adapt the intensity of the applied force.
The maximum force applied with the instrument, which corresponds to the necessary value for the paw's withdrawal, is saved into the system. The operator can then decide to validate the measured value with a simple click on the touchscreen (see screenshot below) or re-launch the measurement with a new stimulation.
Integrated video !
On the EVF5 model, the paw and plantar surface can now be visualized in a high-definition video on the screen of a touchscreen or computer. This method is not only more accurate, it is also much more comfortable for the operator.
: specially designed for touchscreen tablets and offering animal lists management, real-time display of force curves over time, validation of results...
A lighter stimulator
handle with the center of gravity placed more centrally for more precise movement and better control of the increase in force.
The new force sensor is both more accurate and rigid
, improving reproducibility from amore homogenous stimulation, reproducibility. The variation linked to sudden movements is drastically reduced. The system is also easier to use on mice.
New spring tips for mouse stimulation
: by absorbing impact forces, they increase accuracy on the 0-20 grams range.
As well as all the classical functionalities that made our Electronic Von Frey a "must have" for all your research projects in analgesia and nociception:
Large backlit LCD screen simultaneously displaying current and peak value
Statistical functions: average value and standard deviation are computed for each subject
Stand-alone instrument: internal memory allows the storing of up to 100 values, which may be transferred to a PC using our optional software
The brand-new EVF5 software by Bioseb is built around 3 tabs for an optimal ease-of-use while offering numerous innovative functionalities:
Video with target pointer
: No more stretching or bending to target the paw. Real-time video display.
Real-time curve display
: : Immediate display of applied force helps achieve reproducible measures.
Value assignation to each animal's paw
: Special feature for touch screen PC ! Validation in 1 touch (or 1 click).
Animal list management
Exporting results to Microsof Excel
Replay functionalities for both videos and force curves for accurate measurement validation
Measurement validation table with stimulated paw selection
Real-time force over time curves display
Electronic Von Frey is designed to:
Measure the force applied on your subject during stimulation.
Measures are automatically displayed in grams (g)
You can also choose : Newton, lbs and Oz
Measurement from 1 to 500g ! Perfect for any application !
Quickly determine the mechanical pain sensitivity threshold !
1 transportation case
1 foot switch for hand-free resetting of the test
10 disposable measurement tips of 0,5 mm diameter
1 spring tip for thresholds of 0-10 grams