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ACQUISITION SOFTWARE:
ACTITRACK
(Model: ACTITRACK)
Together with IR ACTIMETER LE8811, this software controls data acquisition for one to 32 LE8815 systems and process data analysis

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Other products / accessory  
  • INSERM Faculté de médecine Lille, France
  • Mouse clinic Illkirch, France
  • CRSSA LA TRONCHE, France
  • KEY'OBS Orleans, France
  • COLLEGE DE FRANCE PARIS, France
  • INSERM FAC DE MEDECINE STRASBOURG, France
  • UCB PHARMA BRAINE L'ALLEUD, Belgique
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! NEW RESEARCH WORK ! A recent publication by O Vassal, P Del Carmine, F-P Desgranges, L Bouvet, M Lilot, N Gaot, Q Timour-Chah, D Chassard in "Pain Medicine" highlights the merits of using Bioseb's Acquisition software: ACTITRACK: Assessment of Neurological Toxicity of Hydroxyethyl Starch 130/0.4 Injected in the Intrathecal Space in Rats

Assessment of Neurological Toxicity of Hydroxyethyl Starch 130/0.4 Injected in the Intrathecal Space in Rats
O Vassal, P Del Carmine, F-P Desgranges, L Bouvet, M Lilot, N Gaot, Q Timour-Chah, D Chassard
Department of Anesthesia, H™pital Femme Mere Enfants (Hospices Civils de Lyon), Lyon, France
Published in "Pain Medicine" (2018-02-16)


Objective Epidural blood patch is the procedure of choice to relieve postdural puncture headache. Hydroxyethyl-starch (HES) has been proposed as a patch in some circumstances such as in the case of hematological disease due to the theoretical risk of neoplastic seeding to the central nervous system. Acute neurological HES toxicity has been excluded by a previous animal study, but the long-term neurological toxicity has not been evaluated.
Methods Rats were randomly assigned to one of three groups: no intrathecal injection, 20__L of intrathecal saline, or a 20-_L intrathecal HES (6% hydroxyethyl starch 130/0.4) administered via a cervical puncture. Clinical daily rat activity was measured before and after dural puncture by actinometry. The rats were killed at day 28, and the spinal cord was surgically removed and stained with hematoxylin-phloxine-saffron for gross and microscopic examination.
Results Eleven rats underwent dural puncture without injection, 11 were injected with normal saline, and 12 received intrathecal HES. No clinical or actimetric changes (total distance traveled, number of direction changes, and number of rearings) were observed up to one month after injection. Nonspecific histopathological changes were equally observed in all groups.
Conclusions The results of the current study indicate that intrathecal injection of HES in rats does not induce any clinical or histopathological evidence of long-term neuronal toxicity. Further safety studies in animals are warranted before HES might be considered a safe alternative to the classic epidural blood patch.
Introduction

ActiTrack controls data coming from up to 32 IR frames. It discriminates and analyses frequency and number of IR beam breaks to convert it in a track file that can be analysed later for generating reports. Thus ActiTrack provides much more integrated and precise data in terms of animal activity, position, displacements and rearings than those provided by the SEDACOM software.

Operating principle

The IR actimeter enclosure can be divided into zones of interest (up to 32) using the specific tools provided by Actitrack. Animal trajectories are acquired from real time beam breaks and stored enabling the user to analyze and re-analyse experiments with different zone configurations, associations (user-defined combination of zones) and parameters. Track can be replayed in real-time or by using an "accelerated" (user-defined speed) or "instantaneous" modes.

All data obtained by Actitrack is expressed in ASCII format and can be easily exported to a wide range of spreadsheets and statistics programs for further analysis. Actitrack can be installed in as many computers as required, and the user will be able to check data, generate reports, print out results and obtain charts for visual presentation.

Parameters Measured

• Travelled distance (and % ) into user-defined zones
• Max., min. and mean speed
• Time (and %) moving fast, slow and resting
• Permanence time (and %) into user-defined zones
• Number of Entrance into user-defined zones
• Number and mean duration of rearings
• Number of Clockwise and counter-Clockwise turns
• Track history analysis

Domains of application

• ANXIETY:
- The most prevalent of the psychiatric diseases
- Anxiety disorders

• LOCOMOTOR ACTIVITY AND EXPLORATION:
- Spontaneous activity
- Parkinson’s disease
- Huntington’s disease
- Hyperactivity syndrome


Key features

• Control up to 32 frames
• Provides integrated data (spatial position, pattern of displacement, rearings)
• User-adjustable thresholds for classifying activity into fast, slow and resting movements
• Allows track re-analysis with an unlimited number of user-defined zones
• Enables re-playing experiment using different threshold for movement speed definition
• Can be installed in as many computers as may be required for track analyses

Publications (Click on an article to show details and read the abstract)

CENTRAL NERVOUS SYSTEM (CNS)
- Spinal Cord -
Assessment of Neurological Toxicity of Hydroxyethyl Starch 130/0.4 Injected in the Intrathecal Space in Rats (2018)
Assessment of Neurological Toxicity of Hydroxyethyl Starch 130/0.4 Injected in the Intrathecal Space in Rats
O Vassal, P Del Carmine, F-P Desgranges, L Bouvet, M Lilot, N Gaot, Q Timour-Chah, D Chassard
Department of Anesthesia, H™pital Femme Mere Enfants (Hospices Civils de Lyon), Lyon, France
Published in "Pain Medicine" (2018-02-16)

Objective Epidural blood patch is the procedure of choice to relieve postdural puncture headache. Hydroxyethyl-starch (HES) has been proposed as a patch in some circumstances such as in the case of hematological disease due to the theoretical risk of neoplastic seeding to the central nervous system. Acute neurological HES toxicity has been excluded by a previous animal study, but the long-term neurological toxicity has not been evaluated.
Methods Rats were randomly assigned to one of three groups: no intrathecal injection, 20__L of intrathecal saline, or a 20-_L intrathecal HES (6% hydroxyethyl starch 130/0.4) administered via a cervical puncture. Clinical daily rat activity was measured before and after dural puncture by actinometry. The rats were killed at day 28, and the spinal cord was surgically removed and stained with hematoxylin-phloxine-saffron for gross and microscopic examination.
Results Eleven rats underwent dural puncture without injection, 11 were injected with normal saline, and 12 received intrathecal HES. No clinical or actimetric changes (total distance traveled, number of direction changes, and number of rearings) were observed up to one month after injection. Nonspecific histopathological changes were equally observed in all groups.
Conclusions The results of the current study indicate that intrathecal injection of HES in rats does not induce any clinical or histopathological evidence of long-term neuronal toxicity. Further safety studies in animals are warranted before HES might be considered a safe alternative to the classic epidural blood patch.

OTHER DISORDERS
- Cognitive performance -
Role of cortical microbleeds in cognitive impairment: InÊvivo behavioral and imaging characterization of a novel murine model (2018)
Role of cortical microbleeds in cognitive impairment: InÊvivo behavioral and imaging characterization of a novel murine model
S Bergeron, Y Chen, F Auger, J Deguil, n Durieux, E Skrobala, R Barus, C Potey, C Cordonnier, F Pasquier, L Ravasi, R Bordet, S Gautier
Univ. Lille, Inserm, CHU Lille, Degenerative and Vascular Cognitive Disorders, Lille, France
Published in "Journal of Cerebral Blood Flow & Metabolism" (2018-01-15)

Cerebral microbleeds (CMBs) could contribute to cognitive impairment in the general population and in patients with dementia. We designed a study to (i) develop a murine model of CMBs, (ii) assess whether CMBs affect cognition in this model and (iii) assess whether this model is sensitive to pharmacological modulation. Male C57Bl6/J mice were stereotactically administered collagenase to induce cortical lesion analysed by MRI at 24_h. CMB-mice were assessed at six weeks post-lesion for cognitive performances (Barnes maze and Touchscreen automated paired-associated learning (PAL) task) and for cerebral metabolism (inÊvivo PET/CT with fluorodeoxyglucose (FDG)). CMB-model sensitivity to pharmacological modulation was assessed by administering atorvastatin (5_mg/kg/day) over the follow-up period. CMB mice were compared to na•ve littermates. Collagenase at 0.8_µU/µl appeared suitable to induce reproducible and reliable CMBs. At six weeks, a decline in learning, spatial and visuospatial memory was significantly observed in CMB-mice. Brain metabolism was impaired in all cortex, striatum and the ipsilateral dentate gyrus. A significant improvement in cognition performances was depicted under atorvastatin. In this novel murine model of CMBs, we validated that CMBs lowered cognitive performances and affected regional metabolism. We also proved that this CMB-model is sensitive to pharmacological modulation.


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Computer requirements 2.2 GHz Processor or higher (Celeron processor excluded), 2 Gb of RAM, 150 MB HD, Graphics 1024x768 pixels and 32-bit true colour
System requirements Windows 7 compatible operating system
Communication ports 1 free USB port for the License key
1 RS232 port or additional USB port for the connection to the boxes

Model:
ACTITRACK
Acquisition software: ACTITRACK
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Related products:
SEDACOM
SEDACOM:
SErial DAta COMmunication Software
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LE8815
Infrared Actimeter
IR frame, 450 x 450 mm 16 x 16 IR beams Contact us
Print version

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