Site Metrics and Web Analytics by WebSTAT
Home > Catalog > Learning - Memory - Attention - Addiction
Product
Category
BLACK & WHITE TEST BOX
(Model: LE816 - for mouse - with load cell)
Comparison of anxiolytic drug-induced ease of exploratory activity between an illuminated compartment and a dark one: this test is a quick and evaluation of the animal anxious behavior and its modification by pharmacological agents, and available for rats and mice

- More information - - Documents - - Contact Bioseb -
Users
Publications
Technical specifications
Other products / accessory  
  • IGBMC STRASBOURG, France
  • IBDM MARSEILLE, France
Download PDF
Request video

This page is available in following languages:
! NEW RESEARCH WORK ! A recent publication by M. Leger, V. Bouet, T. Freret, A.-S. Darmaillacq, M. Dacher et al. in "Behavioural Brain Research" highlights the merits of using Bioseb's Black & White Test Box: Environmental enrichment improves recent but not remote memory in association with a modified brain metabolic activation profile in adult mice.

Environmental enrichment improves recent but not remote memory in association with a modified brain metabolic activation profile in adult mice.
M. Leger, V. Bouet, T. Freret, A.-S. Darmaillacq, M. Dacher et al.
Université de Caen Basse-Normandie, Groupe Mémoire et Plasticité comportementale (GMPc), Caen, France.
Published in "Behavioural Brain Research" (2012-03-01)


Environmental enrichment is known to improve learning and memory in adult rodents. Whereas the morphological changes underlying these beneficial effects are well documented, few studies have addressed the influence of this housing condition on the neuronal networks underlying memory processes. We assessed the effects of environmental enrichment on behavioural performances and brain metabolic activation during a memory task in mice. Adult mice were housed in standard (SC) or enriched (EC) conditions for 3 weeks. Then, recent and remote memory performances were measured in the passive avoidance test. After testing, brain metabolic activation was assessed through cytochrome oxidase (CO) activity. EC improved recent memory, in association with an increased metabolic activation in the frontal and prefrontal cortices and a decreased activation in the baso-lateral amygdala and the hippocampus. EC did not improve remote memory, and globally decreased CO activity. Our findings suggest the involvement of regions of pivotal importance during recent memory, such as the frontal cortex, in the beneficial effects of EC.
Black & White (Dark and Light) Test Box for rats and mice by Bioseb - System overview
Black & White Test Box: System overview
Presentation

Numerous behavioural paradigms using several conflict procedures, social interaction or exploration of novel environments have been proposed as animal models of anxiety. B. Costall et al have described (Pharmacol. Biochem. Behav. 32(3): 777-785, 1989) a new model based on the adversive properties of the open field and in which anxiolytic drug-induced ease of exploratory activity is compared between an illuminated compartment and a dark one.

Our dark-light (or Black & White) box allows easy and quick evaluation of the animal anxious behavior and its modification by pharmacological agents by assessing the animal displacements in two compartments with different size, color and illumination. It is a available for both rats and mice

Operating principle

The dark-light experimental box, constructed of Perspex, is composed of a small black compartments and a big white compartment separated by a connecting gate. Each compartment has its own removable Perspex floor of the same color of the respective walls and 90 X 90 mm sectors delimited by lines. The compartments are independently illuminated: the white one with a 100 W white bulb and the black one with a 40 W red bulb. Both bulbs are 370 mm from the floor of the box.

The dark-light box can be supplied with a weight transducer system for automated animal detection. In that case, the automated experimental chambers (up to 8) are associated to the PC-based control software PPCWIN for data storage and analysis.

Even though it may represent a more complex experiment to set due to the strong lighting contrast existing between the compartments, some researchers also use the box with a video tracking system.

Main functionalities

• Compartments with independent and highly contrasted illumination
• Associated with weight transducer technology for optimal animal detection
• Easy to clean between two trials
• Easy connection to a PC through RS232 port
• Exploration-based conflict task
• Based on innate behavioral tendencies (ethological test)
• Light versus dark compartment choice
• Widely used in literature
• Simple to set up and use
• Short-lasting experiment (no more than 5-10 min)
• Sensitive for both rats and mice
Parameters measured

• Total time spent in each compartment
• Latency to the first change of compartment (regardless to the animal initial position)
• Number of changes between the black and white compartments
• Total duration of the experiment

Domains of application

• Anxiety
• Drugs screening
• Phenotyping
• Behavioral/ethological studies

Options

Models LE816 and LE818 have a load cell in each compartment to detect the position of the experimental subject. All models are controlled by the PPCWIN Software

Dedicated PPCWIN Software

The PPCWIN Software can control from one up to eight cages.
It is not necessary the use of any device as interface, since the link is made by serial way and RS232 port (USB)
The Software has the following characteristics:
• Windows™ XP (SP2 or higher), Vista 32, Windows 7 or 8 compatible
• Trial length and Start/Stop are defined separately for each Cage
• Records total time spent in each compartment, for trial
• Records number of transitions between the two areas
• Records latency time before first transition
• Records number of line crossing in the two compartments (activity)
• Once a trial is over, ASCII files containing all information are generated



Up to 8 systems may be controlled simultaneoulsy by the PPCWIN Software

Once a trial is over, ASCII files containing all information are generated.

Publications (Click on an article to show details and read the abstract)


THE INFORMATION IN THIS WEB SITE AND IN LINKED PAGES AND DOCUMENTS IS PROVIDED "AS IS" AND DOES NOT CREATE ANY EXPRESS OR IMPLIED WARRANTY ABOUT BIOSEB OR ITS PRODUCTS OR SERVICES.

Information published on this Web Site as well as services, product specifications, availability and prices are subject to change without notice. BIOSEB may also make improvements and/or changes in the products and/or the programs described in this Web Site at any time without notice.

BIOSEB has made reasonable efforts to verify that the information in this Web site was accurate when first published. Such information may contain errors or omissions, however, and it is subject to change without notice. Bioseb does not undertake to update this information to include any such changes or to correct errors or omissions. Bioseb assumes no responsibility for any use of the information in this Web site or for any infringement of patents or other rights of third parties that may result. Certain information may be country-specific and may not apply in all countries.

Models Specie Dimensions
Black compartment
Dimensions
White Compartment
Dimensions
Shared Wall
Dimensions
Opening
LE810 mouse 16x25x24 cm 25x25x24 cm 47x25 cm 7x7 cm
LE816
(with load cell)
mouse 16x25x24 cm 25x25x24 cm 47x25 cm 7x7 cm
LE812 rat 20x31x24 cm 31x31x24 cm 56x31 cm 10x10 cm
LE818
(with load cell)
rat 20x31x24 cm 31x31x24 cm 56x31 cm 10x10 cm

Model:
LE816
Black & White Test Box
for mouse - with load cell Contact us

Related products:
LE812
Black & White Test Box
for rat Contact us
LE818
Black & White Test Box
for rat - with load cell Contact us
LE810
Black & White Test Box
for mouse Contact us

Accessories :
PPCWIN
PPCWIN software
Contact us
Print version

Bioseb - In Vivo Research Instruments
Phone worldwide : +33 442 344 360 - USA/Canada : (727) 521-1808
e-Mail : Worldwide: info@bioseb.com - USA/Canada: info-us@bioseb.com