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STARTLE AND FEAR SYSTEM
(Model: LE116 - For rats and mice)
A polyvalent system for conducting both fear conditioning and startle reflex experiments

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! NEW RESEARCH WORK ! A recent publication by L Khrimian, A Obri, M Ramos-Brossier, A Rousseaud, S Moriceau, A-S Nicot, P Mera, S Kosmidis, T Karnavas, F Ssudou, X-B Gao, F Oury, E Kandel, G Karsenty in "Journal of Experimental Medicine" highlights the merits of using Bioseb's Startle and Fear System: Gpr158 mediates osteocalcinŐs regulation of cognition

Gpr158 mediates osteocalcinŐs regulation of cognition
L Khrimian, A Obri, M Ramos-Brossier, A Rousseaud, S Moriceau, A-S Nicot, P Mera, S Kosmidis, T Karnavas, F Ssudou, X-B Gao, F Oury, E Kandel, G Karsenty
Department of Genetics and Development, Columbia University Medical Center, New York, NY
Published in "Journal of Experimental Medicine" (2017-08-29)


That osteocalcin (OCN) is necessary for hippocampal-dependent memory and to prevent anxiety-like behaviors raises novel questions. One question is to determine whether OCN is also sufficient to improve these behaviors in wild-type mice, when circulating levels of OCN decline as they do with age. Here we show that the presence of OCN is necessary for the beneficial influence of plasma from young mice when injected into older mice on memory and that peripheral delivery of OCN is sufficient to improve memory and decrease anxiety-like behaviors in 16-mo-old mice. A second question is to identify a receptor transducing OCN signal in neurons. Genetic, electrophysiological, molecular, and behavioral assays identify Gpr158, an orphan G proteinĐcoupled receptor expressed in neurons of the CA3 region of the hippocampus, as transducing OCNŐs regulation of hippocampal-dependent memory in part through inositol 1,4,5-trisphosphate and brain-derived neurotrophic factor. These results indicate that exogenous OCN can improve hippocampal-dependent memory in mice and identify molecular tools to harness this pathway for therapeutic purposes.

Presentation

The "Startle and Fear" system is a polyvalent system taking advantage of the same equipment for conducting both fear conditioning and startle reflex experiments, regardless the animal is a rat or a mouse.


Operating principle

Basically, the "Startle and Fear" system allows the recording and the analysis of the signal generated by the animal movement through a high sensitivity Weight Transducer system. The analogical signal is transmitted to the STARTLE or FEAR software through the load cell unit for recording purposes and posterior analysis in terms of activity/immobility (fear conditioning) or startle response characterization (startle reflex). Two additional control units allow setting the frequency and the amplitude of the sound (pre-pulse, pulse, background noise) as well as the intensity of the shock.

The "Startle and Fear" cage is made with black methacrylate walls and a transparent frond door. In fear conditioning experiment, the walls, cover and floor can be of different material or colour. Moreover, a transparent cylinder can be placed into the experimental chamber in order to modify the contextual spatial perception of the subject during the test phase.


Parameters Measured

All the parameters given by the Startle or fear software, i.e.:
• Time of experiment at which each activity event has occured (fear conditioning)
• Duration of each inactivity event (fear conditioning)
• Latency until the maximum amplitude of startle response (startle reflex)
• Duration of the startle response (startle reflex)
• Latency until the begining of the startle response (startle reflex)
• Average of the startle response (startle reflex) Accessories Startle and Fear


Key features

• Combined system for Startle/Freezing and Rat/Mice
• Unmatched sensitivity for mice
• One same enclosure for either rat or mouse
• Same weight transducer platform regardless the weight of the animal (15-500g)
• Hightly flexible protocol editor
• Accurate and entirely traceable data
• Tone frequency and intensity controlled by software
• Easily removable tray
• Different spatial context configurations available for fear conditioning paradigms
• No PCI cards required


Supplied with

• Experimental Chamber
• Stimulation Unit
• Sound Proof Box (LE26)
• Weight cell amplifier (LE111)
• Audio Generator (LE118)


Options

• Mouse/Rat holder for Startle Reflex (LE117M or LE117R)
• Shock Generator (LE10026)
• StartWin Software to control up to 8 Stimulation units


Publications (Click on an article to show details and read the abstract)

MOOD DISORDERS
- Anxiety -
Gpr158 mediates osteocalcinŐs regulation of cognition (2017)
Gpr158 mediates osteocalcinŐs regulation of cognition
L Khrimian, A Obri, M Ramos-Brossier, A Rousseaud, S Moriceau, A-S Nicot, P Mera, S Kosmidis, T Karnavas, F Ssudou, X-B Gao, F Oury, E Kandel, G Karsenty
Department of Genetics and Development, Columbia University Medical Center, New York, NY
Published in "Journal of Experimental Medicine" (2017-08-29)

That osteocalcin (OCN) is necessary for hippocampal-dependent memory and to prevent anxiety-like behaviors raises novel questions. One question is to determine whether OCN is also sufficient to improve these behaviors in wild-type mice, when circulating levels of OCN decline as they do with age. Here we show that the presence of OCN is necessary for the beneficial influence of plasma from young mice when injected into older mice on memory and that peripheral delivery of OCN is sufficient to improve memory and decrease anxiety-like behaviors in 16-mo-old mice. A second question is to identify a receptor transducing OCN signal in neurons. Genetic, electrophysiological, molecular, and behavioral assays identify Gpr158, an orphan G proteinĐcoupled receptor expressed in neurons of the CA3 region of the hippocampus, as transducing OCNŐs regulation of hippocampal-dependent memory in part through inositol 1,4,5-trisphosphate and brain-derived neurotrophic factor. These results indicate that exogenous OCN can improve hippocampal-dependent memory in mice and identify molecular tools to harness this pathway for therapeutic purposes.

OTHER DISORDERS
- Cognitive performance -
Gpr158 mediates osteocalcinŐs regulation of cognition (2017)
Gpr158 mediates osteocalcinŐs regulation of cognition
L Khrimian, A Obri, M Ramos-Brossier, A Rousseaud, S Moriceau, A-S Nicot, P Mera, S Kosmidis, T Karnavas, F Ssudou, X-B Gao, F Oury, E Kandel, G Karsenty
Department of Genetics and Development, Columbia University Medical Center, New York, NY
Published in "Journal of Experimental Medicine" (2017-08-29)

That osteocalcin (OCN) is necessary for hippocampal-dependent memory and to prevent anxiety-like behaviors raises novel questions. One question is to determine whether OCN is also sufficient to improve these behaviors in wild-type mice, when circulating levels of OCN decline as they do with age. Here we show that the presence of OCN is necessary for the beneficial influence of plasma from young mice when injected into older mice on memory and that peripheral delivery of OCN is sufficient to improve memory and decrease anxiety-like behaviors in 16-mo-old mice. A second question is to identify a receptor transducing OCN signal in neurons. Genetic, electrophysiological, molecular, and behavioral assays identify Gpr158, an orphan G proteinĐcoupled receptor expressed in neurons of the CA3 region of the hippocampus, as transducing OCNŐs regulation of hippocampal-dependent memory in part through inositol 1,4,5-trisphosphate and brain-derived neurotrophic factor. These results indicate that exogenous OCN can improve hippocampal-dependent memory in mice and identify molecular tools to harness this pathway for therapeutic purposes.


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Soundproof box dimensions 670 (W) x 530 (D) x 550 (H) mm
Cage dimensions 250 (W) x 250 (D) x 250 (H) mm
Material Composition Methacrylate, aluminium, stainless steel
Maximum number of stations 8 stations connected to a PC
Sound frequency and amplitude Prepulse/Pulse: adjustable from 200 to 10000 Hz Max 120 dB,
White noise from 60 to 120 dB
Certifications CE Compliant
Power Supply 110V/220V, 50/60Hz

Model:
LE116
Startle and Fear System
For rats and mice Contact us

Related products:
STARTLE
Startle Software
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FREEZING
FREEZING software
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Print version

Bioseb - In Vivo Research Instruments
Phone worldwide : +33 442 344 360 - USA/Canada : (727) 521-1808
e-Mail : Worldwide: info@bioseb.com - USA/Canada: info-us@bioseb.com