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RODENT PINCHER - ANALGESIA METER
(Model: BIO-RP-R - For rats)
A fast and accurate algometer based on an instrumented pincher as an alternative to the Randall-Selitto test: This new analgesia meter developed by Bioseb following Luis-Delgado et al. (2005) allows accurate nociceptive tests to measure mechanical pain threshold on rat or mouse limbs with minimal constraint.

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  • UNIVERSITY OF MANITOBA Winnipeg, Canada
  • VERTEX PHARMACEUTICALS San Diego, USA
  • DUKE UNIVERSITY MEDICAL CENTER Durham, USA
  • MC MASTER UNIVERSITY Hamilton, Canada
  • PFIZER Sandwich, UK
  • INNSBRUCK MEDICAL UNIVERSITY Innsbruck, Austria
  • HARVARD MEDICAL SCHOOL Charlestown, USA
  • UNIL Lausanne, Switzerland
  • CENTRE HOSPITALIER ESQUIROL Limoges, France
  • CNRS UMR 7519 Strasbourg, France
  • ESCPI Paris, France
  • TROPHOS Marseille, France
  • INSERM U 788 Kremlin Bicêtre, France
  • INSTITUT FRANCOIS MAGENDIE Bordeaux, France
  • LABO DE PHYSIOLOGIE ET MANDUCATION Paris, France
  • PHARMALEADS Paris, France
  • SANOFI AVENTIS Vitry Sur Seine, Porcheville, Montpellier & Toulouse, France
  • CNRS, IPMC Valbonne, France
  • BIOCODEX Compiègne, France
  • INSTITUT PASTEUR Paris, France
  • CRSSA La Tronche, France
  • INSTITUT HENRY BEAUFOUR Les Ulis, France
  • PORSOLT AND PARTNERS Le Genest ST Isle, France
  • ASTRAZENECA R&D MONTREAL St Laurent, Canada
  • MEDICAL UNIVERSITY OF VIENNA VIENNA, AUSTRIA
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! NEW RESEARCH WORK ! A recent publication by J Wahis, D Kerspern, F Althammer, A Baudon, S Goyon et al in "bioRxiv" highlights the merits of using Bioseb's Rodent pincher - analgesia meter: Oxytocin Acts on Astrocytes in the Central Amygdala to Promote a Positive Emotional State

Oxytocin Acts on Astrocytes in the Central Amygdala to Promote a Positive Emotional State
J Wahis, D Kerspern, F Althammer, A Baudon, S Goyon et al
INCI, CNRS UPR3212 8, Allée du Général Rouvillois, 67000 Strasbourg, France
Published in "bioRxiv" (2020-02-26)


Oxytocin orchestrates social and emotional behaviors through modulation of neural circuits in brain structures such as the central amygdala (CeA). The long-standing dogma is that oxytocin signaling in the central nervous system occurs exclusively via direct actions on neurons. However, several findings over the last decades showed that astrocytes actively participate in the modulation of neuronal circuits. Here, we investigate the degree of astrocytes’ involvement in oxytocin functions. Using astrocyte’ specific gain and loss of function approaches, we demonstrate that CeA astrocytes not only directly respond to oxytocin, but are actually necessary for its effects on neuronal circuits and ultimately behavior. Our work identifies astrocytes as a crucial cellular substrate underlying the promotion of a positive emotional state by oxytocin. These results further corroborate that astrocytes are key regulators of neuronal circuits activity by responding to specific neuropeptidergic inputs, and opens up new perspectives to understand how neuromodulators gate brain functions.
Presentation

 Changes of the nociceptive hind paw threshold induced by morphine Bioseb's "Rodent Pincher" algometer allows calibrated forceps to induce quantifiable mechanical stimulation in the animal (rats or mice) on a linear scale. The most suitable protocol was experimented by determining the effects of 3 repetitive measurements on the 2 hindpaws, respectively over long-term (9 days), mid-term (1 day) and short-term (2 hours). It was primarily developed as an analgesimeter/algometer (analgesia measurement instrument) for nociceptive tests. In this specific use, the Rodents Pincher not only represents an alternative to the "RANDALL & SELITTO" test - but also presents the following advantages when compared to the classical test:
• More ethical handling, less stressful for the animal (rat or mouse) resulting in less variable measurements
• Faster measurement, less traumatic for the tissues
• Digital measurement with metrological traceability

Furthermore, this pincher-based analgesia meter can also be used for other applications that require a controlled force or pressures values, for example :
• Sensitivity recovery after nerve crush: pinching is applied at several places over the paw to see the sensitivity recovery
• Mechanical injuries for thrombotic models
• Mechanical stimulation for in vivo electrophysiological recordings using the possibility to trigger recording on force thresholds

Comparative studies (including comparative tests with the Randall & Sellito analgesimeter) have demonstrated the accuracy of this pincher-based algometer for easy, fast and reproducible measurement of mechanical pain threshold on rat limbs. Moreover, it allowed to perform rat analagesia testing with minimal constraint, which reduces data variability.


One of these experimental research works was carried out by the team of Prof. Poisbeau in the "Nociception & Pain" department of the "Institut des Neurosciences Cellulaires et Intégratives" (Université Louis pasteur) in Strasbourg.

This work was described in two articles published in December 2005 in international reviews with a controlling committee:

Validation method :
Calibrated Forceps: A Sensitive and Reliable Tool for Pain and Analgesia Studies, by Luis-Delgado et al. in The Journal of pain, December 2005

Scientific work :
Inflammatory Pain Upregulates Spinal Inhibition via Endogenous Neurosteroid Production, by P. Poisbeau et al. in The Journal of neuroscience, December 2005

Click on the title of one of the articles to send a request to download the PDF file

Operating principle

Bioseb's Rodent Pincher Analgesimeter: Embedded statistical computation Bioseb's Rodent Pincher Analgesimeter: Embedded statistical computation The pressure is applied in the "inter-digital" region. When used on the rat the pressure can also be applied to the tail. The instrument displays the force (in grams, newton, oz, lbs) at which the animal reacts and reports the nociception threshold.

Individual pain threshold measurements (up to 100) are stored in the internal memory, and can be downloaded post experiment. Recently an embedded statistical computation has been included in the electronic device of the algometer. This is a very useful feature that as been very well received and used by users of large numbers of tests. The display shows in real time the mean, standard deviation and variation coefficient from groups of animals (rats or mice). This feature also allows the user to cancel any analgesia test incorrectly performed.


Parameters measured

The following parameters are measured by Bioseb's rodents pincher / analgesia instrument:
• Maximum force applied as the animal reacts (movement, vocalization) (in grams, N, oz, lbs)
• Duration of the test (using Bioseb's BIO-CIS software) in seconds


Domains of application

Bioseb's Rodent Pincher Algometer can be used for a variety of research on nociception and analgesia, including:
• In vivo models evaluation of pain
• Mechanisms related to mechanical nociception
• Whenever a calibrated force is necessary

Bioseb's new pincher design: mounting of custom-made bits to suit your most specific needs and analgesia applications
Built-in components

These built-in components will make the analgesia measurement easier:
• Desktop stand for easy reading
• Footswitch to reset the zero of the rodent pincher analgesimeter while keeping hands free.
• RS232 output to transfer displayed data to a PC.
• Our new pincher design allows the mounting of custom-made bits to suit your most specific needs and applications. Please contact us if you have any special request about the pincher, and we'll do our best to cover your needs.


Options

• Our optional BIO-CIS software for Windows sends directly the displayed values in an Excel (MS) sheet. This allows the user direct access to a ready report and options for further analysis, without any re-copy error. Supplied with an RS232 cable.
• External pod to display a led light or produce a sound when reaching a preset threshold of force.
• Analog output and cable
• TTL output
• Additional sensor tests : our Electronic Von Frey probe, Small Animal Algometer come with their own calibration modules that are recognized by the electronic unit.


Supplied with

• Bench control unit (batteries and main power supply)
• One rat or mice pincher, capacity 2000 grs
• Carrying case
• Footswitch for zeroing the force and having the hands "free"
• RS232 output
• User manual

New system! AlgoKit

AlgoKit Algometer, by Bioseb
AlgoKit
A COMPLETE SOLUTION FOR ALL SITUATIONS
INVOLVING PAIN MEASUREMENT ON RODENTS
MULTIPLE PROBES, ONE CONTROL UNIT AND SOFTWARE APPLICATION

You may consider our new ALGOKIT package to expand the scope of your possibilities. In order to offer an answer to all your requests regarding different situations of pain measurement, Bioseb is proud to present an innovative, complete solution combining our Static Weight Bearing, including its state-of-the art touchscreen, color console, with up to 3 sensors to be chosen among our Electronic Von Frey, Rodent Pincher and Small animal Algometer, all connected to one unique Control Unit.

Features:
• Flexible instrument when a quantitative sensory testing (QST) is needed
• Compatible with our BIO-CIS software to improve repeatability in nociceptive testing


Publications (Click on an article to show details and read the abstract)

PAIN
- General pain -
Effect of 1-Hydroxy-1,1-thylidendiphosphone Acid, Bis(2-Pyridyl-1,2,4-Triazolyl-3)Propane and their adduct on the pain sensitivty of Rats (2019)
Effect of 1-Hydroxy-1,1-thylidendiphosphone Acid, Bis(2-Pyridyl-1,2,4-Triazolyl-3)Propane and their adduct on the pain sensitivty of Rats
IV Cheretaev, MY Ravaeva, ER Dzheldubaeva et al
V. I. Vernadsky Crimean Federal University, Simferopol, Crimea, Russia
Published in "Biology Chemistry" (2019-07-01)

The aim of the work is to evaluate the effect of 1-hydroxy-1,1ethylidenediphosphonic acid, bis(2-pyridyl-1,2,4-triazolyl-3)propane and their adduct on the pain sensitivity of male rats in the dose range from 5 to 200 mg/kg.

INFLUENCE OF 1-HYDROXY-1,1-ETHYLIDENDIPHOSPHONE ACID AND BIS (2-PYRIDYL-1,2,4-TRIAZOLYL-3) PROPANE ON PAIN SENSITIVITY OF RAT FEMALES (2019)
INFLUENCE OF 1-HYDROXY-1,1-ETHYLIDENDIPHOSPHONE ACID AND BIS (2-PYRIDYL-1,2,4-TRIAZOLYL-3) PROPANE ON PAIN SENSITIVITY OF RAT FEMALES
IV Cheretaev, EN Chuyan, MY Ravaeva, VF Shulgin
Tavrida Academy (structural unit) FSAEI of HE, V.I. Vernadsky Crimean Federal University, Simferopol, Russia
Published in "International Research Journal" (2019-07-01)

The paper presents the results of studying the effect of 1-hydroxy-1,1-ethylidene diphosphonic acid and bis(2-pyridyl-1,2,4-triazolyl-3) propane with doses of 5, 50, 100 and 200 mg/kg on the pain sensitivity of rat females. The experiments were conducted on 100 Wistar female rats on acute thermal (tail-flick, hot plate) and mechanical pain test models (Randall-Selitto test). It was found that 1-hydroxy-1,1-ethylidene diphosphonic acid has a clear analgesic effect with thermal effects in doses of 5 and 200 mg/kg, and mechanical pain effects with a dose of 50 mg/kg; bis (2-pyridyl-1,2,4-triazolyl-3) propane in the tail-flick test with doses of 5 and 50 mg/kg, in the hot plate test, with the dose of 50 mg/kg, in the Randall-Selittto test, with a dose of 50 mg/kg.

Cholecalciferol (Vitamin D 3) Reduces Rat Neuropathic Pain by Modulating Opioid Signaling (2019)
Cholecalciferol (Vitamin D 3) Reduces Rat Neuropathic Pain by Modulating Opioid Signaling
P Poisbeau, M Aouad, G Gazzo, A Lacaud, V Kemmel et al
Centre National de la Recherche Scientifique, UniversitŽ de Strasbourg, Institut des Neurosciences Cellulaires et IntŽgratives, Strasbourg, France
Published in "Molecular Neurobiology" (2019-04-18)

The impact of vitamin D on sensory function, including pain processing, has been receiving increasing attention. Indeed, vitamin D deficiency is associated with various chronic pain conditions, and several lines of evidence indicate that vitamin D supplementation may trigger pain relief. However, the underlying mechanisms of action remain poorly understood. We used inflammatory and non-inflammatory rat models of chronic pain to evaluate the benefits of vitamin D3 (cholecalciferol) on pain symptoms. We found that cholecalciferol supplementation improved mechanical nociceptive thresholds in monoarthritic animals and reduced mechanical hyperalgesia and cold allodynia in a model of mononeuropathy. Transcriptomic analysis of cerebrum, dorsal root ganglia, and spinal cord tissues indicate that cholecalciferol supplementation induces a massive gene dysregulation which, in the cerebrum, is associated with opioid signaling (23 genes), nociception (14), and allodynia (8), and, in the dorsal root ganglia, with axonal guidance (37 genes) and nociception (17). Among the identified cerebral dysregulated nociception-, allodynia-, and opioid-associated genes, 21 can be associated with vitamin D metabolism. However, it appears that their expression is modulated by intermediate regulators such as diverse protein kinases and not, as expected, by the vitamin D receptor. Overall, several genesÑOxt, Pdyn, Penk, Pomc, Pth, Tac1, and Tgfb1Ñencoding for peptides/hormones stand out as top candidates to explain the therapeutic benefit of vitamin D3 supplementation. Further studies are now warranted to detail the precise mechanisms of action but also the most favorable doses and time windows for pain relief.

Corticosterone analgesia is mediated by the spinal production of neuroactive metabolites that enhance GABAergic inhibitory transmission on dorsal horn rat neurons (2015)
Corticosterone analgesia is mediated by the spinal production of neuroactive metabolites that enhance GABAergic inhibitory transmission on dorsal horn rat neurons
Zell V, Juif PÉ, Hanesch U, Poisbeau P, Anton F, Darbon P.
Centre National de la Recherche Scientifique and University of Strasbourg, Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France
Published in "Eur J Neurosci. " (2015-02-01)

Corticosterone (CORT) is a glucocorticoid produced by adrenal glands under the control of the hypothalamic-pituitary-adrenal axis. Circulating CORT can enter the central nervous system and be reduced to neuroactive 3?5?-reduced steroids, which modulate GABAA receptors. In the dorsal spinal cord, GABAergic transmission modulates integration of nociceptive information. It has been shown that enhancing spinal inhibitory transmission alleviates hyperalgesia and allodynia. Therefore, the spinal neuronal network is a pivotal target to counteract pain symptoms. Thus, any increase in spinal 3?5?-reduced steroid production enhancing GABAergic inhibition should reduce nociceptive message integration and the pain response. Previously, it has been shown that high levels of plasma glucocorticoids give rise to analgesia. However, to our knowledge, nothing has been reported regarding direct non-genomic modulation of neuronal spinal activity by peripheral CORT. In the present study, we used combined in vivo and in vitro electrophysiology approaches, associated with measurement of nociceptive mechanical sensitivity and plasma CORT level measurement, to assess the impact of circulating CORT on rat nociception. We showed that CORT plasma level elevation produced analgesia via a reduction in C-fiber-mediated spinal responses. In the spine, CORT is reduced to the neuroactive metabolite allotetrahydrodeoxycorticosterone, which specifically enhances lamina II GABAergic synaptic transmission. The main consequence is a reduction in lamina II network excitability, reflecting a selective decrease in the processing of nociceptive inputs. The depressed neuronal activity at the spinal level then, in turn, leads to weaker nociceptive message transmission to supraspinal structures and hence to alleviation of pain.

Characterization of the fast GABAergic inhibitory action of etifoxine during spinal nociceptive processing in male rats (2014)
Characterization of the fast GABAergic inhibitory action of etifoxine during spinal nociceptive processing in male rats
Juif PE, Melchior M, Poisbeau P
Centre National de la Recherche Scientifique and University of Strasbourg, Institute of Cellular and Integrative Neurosciences, Strasbourg, France
Published in "Neuropharmacology. " (2014-12-26)

Etifoxine (EFX) is a non-benzodiazepine anxiolytic which potentiate GABAA receptor (GABAAR) function directly or indirectly via the production of 3?-reduced neurosteroids. The later effect is now recognized to account for the long-term reduction of pain symptoms in various neuropathic and inflammatory pain models. In the present study, we characterized the acute antinociceptive properties of EFX during spinal pain processing in naive and monoarthritic rats using in vivo electrophysiology. The topical application of EFX on lumbar spinal cord segment, at concentrations higher than 30 ?M, reduced the excitability of wide dynamic range neurons receiving non-nociceptive and nociceptive inputs. Windup discharge resulting from the repetitive stimulation of the peripheral receptive field, and recognized as a short-term plastic process seen in central nociceptive sensitization, was significantly inhibited by EFX at these concentrations. In good agreement, mechanical nociceptive thresholds were also significantly increased following an acute intrathecal injection of EFX. The acute modulatory properties of EFX on spinal pain processing were never seen in the simultaneous presence of bicuculline. This result further confirmed EFX antinociception to result from the potentiation of spinal GABAA receptor function.

Plasma glucocorticoids differentially modulate phasic and tonic GABA inhibition during early postnatal development in rat spinal lamina II (2014)
Plasma glucocorticoids differentially modulate phasic and tonic GABA inhibition during early postnatal development in rat spinal lamina II
V. Zella, U. Haneschb, P. Poisbeaua, F. Antonb, P. Darbon
Centre National de la Recherche Scientifique and University of Strasbourg, Strasbourg, France
Published in "Neuroscience Letters" (2014-06-23)

Nociceptive processing is tuned by GABAA receptor-mediated inhibition in the spinal cord dorsal horn that undergoes postnatal maturation in rodents. These GABAergic inhibitory postsynaptic currents (IPSCs) are modulated by 3α5α-reduced steroids during early postnatal development in spinal cord lamina II. Thus an enhanced phasic inhibition is present in neonates and decreases over time. GABA can also activate extrasynaptic receptors, giving rise to tonic inhibition. In this study, we characterized the contribution of plasma corticosterone (CORT) to postnatal maturation of spinal phasic and, for the first time, tonic GABAergic inhibitions. We used Fisher and Lewis rat strains displaying respectively high and low hypothalamic-pituitary-adrenal axis reactivity, compared to control Sprague-Dawley rats. Measured plasma CORT levels were significantly higher in Fisher rats, which also displayed significantly higher mechanical nociceptive thresholds, supporting the hypothesis of an antinociceptive action of CORT. Recorded GABAA IPSCs shortened during maturation in all strains while remaining larger in Fisher rats. Blocking the 5α-reduction of steroids in Fisher rats produced a further decrease of IPSC deactivation time constant. In contrast, GABAA tonic inhibition progressively increased during maturation, without any difference among strains. In conclusion, we show that both phasic and tonic GABAergic inhibitions undergo postnatal maturation in lamina II. Moreover spinal production of 3α5α-reduced steroids that presumably derive from plasma CORT is correlated to spinal GABAA phasic (but not tonic) inhibition and to mechanical nociceptive thresholds.



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Animal Rat or mouse (2 different pinchers)
Measuring Range 0 to 2000 grams
Units Grams / newtons
Resolution 0,1 gram or 0,001 N
Results Displays the current applied force and the maximum applied force during the test
Statistic Mean, standard deviation and variation coefficient
Power supply Battery powered, this instrument is autonomous.
Possibility of using a 220V/110V 50hz/60hz adapter.
Data output RS232 for PC or printer
EMC Controlled

Model:
BIO-RP-R
Rodent pincher - analgesia meter
For rats Contact us

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Accessories :
BIO-CIS
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Print version

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