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TAIL FLICK METER
(Model: LE7106)
Particularly sensitive for studying the analgesic properties of pharmacological substances

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  • NEUROFIT illkirch, France
  • ICS ILLKIRCH, France
  • IGBMC ILLKIRCH, France
  • CEREP PARIS, France
  • SANOFI BAGNEUX, France
  • Actelion Allschwill, suisse
  • CNRS Marseille, France
  • FACULTY OF PHARMACY Limoge, France
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! NEW RESEARCH WORK ! A recent publication by R. Weibel, D. Reiss, L. Karchewski, O. Gardon, A. Matifas et al. in "PLOS One" highlights the merits of using Bioseb's Tail flick meter: Mu Opioid Receptors on Primary Afferent Nav1.8 Neurons Contribute to Opiate-Induced Analgesia: Insight from Conditional Knockout Mice

Mu Opioid Receptors on Primary Afferent Nav1.8 Neurons Contribute to Opiate-Induced Analgesia: Insight from Conditional Knockout Mice
R. Weibel, D. Reiss, L. Karchewski, O. Gardon, A. Matifas et al.
IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université de Strasbourg, Illkirch, France
Published in "PLOS One" (2013-09-12)


Opiates are powerful drugs to treat severe pain, and act via mu opioid receptors distributed throughout the nervous system. Their clinical use is hampered by centrally-mediated adverse effects, including nausea or respiratory depression. Here we used a genetic approach to investigate the potential of peripheral mu opioid receptors as targets for pain treatment. We generated conditional knockout (cKO) mice in which mu opioid receptors are deleted specifically in primary afferent Nav1.8-positive neurons. Mutant animals were compared to controls for acute nociception, inflammatory pain, opiate-induced analgesia and constipation. There was a 76% decrease of mu receptor-positive neurons and a 60% reduction of mu-receptor mRNA in dorsal root ganglia of cKO mice. Mutant mice showed normal responses to heat, mechanical, visceral and chemical stimuli, as well as unchanged morphine antinociception and tolerance to antinociception in models of acute pain. Inflammatory pain developed similarly in cKO and controls mice after Complete Freund’s Adjuvant. In the inflammation model, however, opiate-induced (morphine, fentanyl and loperamide) analgesia was reduced in mutant mice as compared to controls, and abolished at low doses. Morphine-induced constipation remained intact in cKO mice. We therefore genetically demonstrate for the first time that mu opioid receptors partly mediate opiate analgesia at the level of Nav1.8-positive sensory neurons. In our study, this mechanism operates under conditions of inflammatory pain, but not nociception. Previous pharmacology suggests that peripheral opiates may be clinically useful, and our data further demonstrate that Nav1.8 neuron-associated mu opioid receptors are feasible targets to alleviate some forms of persistent pain.

Presentation

Basically, a radiant heat is applied on the tail; when the animal feels discomfort, it reacts by a sudden tail's movement (tail flick) which automatically stops the stimulation and the timer for the measurement of the animal reaction time (period from the beginning of the stimulation until detection of the animal’s response).

This test has proved particularly sensitive for studying the analgesic properties of pharmacological substances. It can also be used to evaluate basal thermal pain sensitivity or to study putative genetic differences among animals without drug ('naïve').


Operating principle

The LE7106 Tail-flick Meter consists of a Stimulation Unit (containing the halogen lamp for the heat stimulus) and an electronic Control Unit. The system can be used for rats and mice of different sizes. The animals are placed with its tail protruding within a restraining tube on the platform of the Stimulus Unit. The animal’s tail is positioned on a slot of adjustable width equipped with a groove that guarantees a correct placement of the rodent’s tail. A remote foot-switch controls the test start/stop allowing rapid hands-free experiments.

A photo beam with adjustable sensitivity detects the tail flick and the latency is automatically presented on a digital display on the Control Unit. Measurements of reaction time are given with a 0.01s precision. A cut-off time can be set to avoid tissue damage (by default: 20 s). The precise control of the heat stimulus and the groove system ensure optimum repeatability and reliability of results.

The optional SeDacom software (new version 2.0 available) can be used and represents an easy and convenient way to visualize and export the data on a computer for further analysis.


Parameters Measured

• Time latency responses to thermal stimulus (tail flick)


Key features

• Digital display
• Precise intensity light beam adjustment
• Optimal detection due to perfect alignment of heat stimulus and photo beam
• A light beam shows the point on which the heat source will focus
• Manual and remote trigger
• Groove for correct tail placement
• Automatic cut-off
• New optional Data Transfer software SEDACOM 2.0


Options

• LE7106T Tail-temperature Recorder
SEDACOM Software
• RS232/USB adapter for USB communication (for SEDACOM)
LE7106 with the new LE7106T temperature recorder
LE7106 with the new LE7106T temperature recorder



Publications (Click on an article to show details and read the abstract)


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Control Unit Dimensions 350 (W) x 350 (D) x 130 (H)
Stimulation Unit Dimensions 400 (W) x 140 (D) x 155 (H)
Power Supply 110V/220V, 50/60Hz
Material Composition Methacrylate, halogen lamp
Computer Requirements PC (Windows 7)
Maximum number of stations 1 per computer (multiple set-ups also available under request)
Certifications CE Compliant

Model:
LE7106
Tail flick meter
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Accessories :
LE5016
Rodents Restrainers
Mice up to 35 g (90 x 30 mm) Contact us
LE5018
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Mice up to 50 g (100 x 34 mm) Contact us
LE5020
Rodents Restrainers
Rats from 80 up to 200 g (150 x 44 mm) Contact us
LE5022
Rodents Restrainers
Rats from 180 up to 320 g (180 x 54 mm) Contact us
LE5024
Rodents Restrainers
Rats from 300 to 440 g (200 x 64 mm) Contact us
LE5025
Rodents Restrainers
Rats from 440 up to 550 g (225 x 74 mm) Contact us
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