! NEW RESEARCH WORK !
A recent publication
by M Bohic, I Marics, C Santos, P Malapert, N Ben-Arie et al in "Cell Reports" highlights the merits of using Bioseb's Thermal Place Preference, 2 Temperatures Choice Nociception Test
: Loss of bhlha9 Impairs Thermotaxis and Formalin-Evoked Pain in a Sexually Dimorphic Manner
Overview of the Temperature Choice Test System
Bioseb’s Thermal Place Preference Test, or 2 Temperatures Choice Nociception Test, is an operator independant test to study pain thresholds in rodents (mouse and rat) by assessing temperature preference (comfort zone) - a brand new tool opening new fields of investigation for your analgesia/nociception research.
As advised by A. MOQRICH, and published in Moqrich et al (Science 2005, 307: 1468-72), the Thermal Place Preference Test allows researchers to work on unrestrained animals (mice and rats) let free to choose their preferred position (comfort zone) between 2 compartments set at different temperatures. This behavioural assay will allow monitoring temperature preferences, nociceptive thresholds and state in the role of a given gene or a compound on these pain thresholds associated to cold and hot stimulation.
Unlike the cold/hot plate test, it is investigator-independent: using the traditional plates, an operator can measure the reaction time of an animal (mouse or rat) exposed to a certain temperature. The "two Temperatures Choice Test" will return a nociceptive response without any action from the operator, and the obtained value is a temperature or a temperature range indicating the sensitivity of the animal (mouse or rat) resulting to the exposure to different stimulations (cold or heat).
You have the possibility to either observe one rat at a time or two mice simultaneously and independently, making the Thermal Place Preference system remarkably attractive for your analgesia research.
This test is easy to setup:
The optional, dedicated T2CT software is a convenient tool allowing the operator to define the temperature of each zone, and easily, automatically obtain the position & presence time of each animal (mouse or rat). Results can be transferred directly as an Excel file or as a txt format. Software is compatible with Windows 7/8, in both 32 and 64 bits, and comes with 3 USB cables and a webcam.
T2CT software v2 is now available!
• New algorithm for tracking activity and detecting zone transitions
• A single window for all settings
• New improved electronics are more stable and result in faster temperature transitions
Parameters measured include :
• Time spent in each temperature zone (abs. and %)
• Time of each zone trespassing
• Temperature of each zone
• Activity time of the animal (total or by zone)
• Distance run by the animal (total or by zone)
• Innovative test opening new fields of investigation
• Easy to set-up
• Operated on unrestrained, freely moving animals
• Automatic if using the optional software
• Windows 7/8 32/64bits compatible
• Transition lists can be managed or imported from xls/csv file
• Ability to resume an interrupted session
• Replay experimental videos, incl. animal detection
• Display and export results for custom time periods
• Customized results exports
• Results grouping for animal groups
• Results can be displayed as table or graph format
Domains of application
• Drug screening
Using the optional automatic detection software: the operator defines the temperature of each zone. Once the temperatures are stabilized, animals are placed and the acquisition can be launched. The operator doesn't have to be present any more during the nociceptive experiment.
If not using the automatic detection software, temperatures have to be defined manually, and the operator has to measure the time himself.
During the thermal analgesia experience, the operator can visualize the position and movements of each animal on the screen. Raw data acquisition files are stored in a proprietary format to match GLP norms, while a .txt version of the file can be used for further analysis in MS Excel.
Automatic detection software to measure the position and presence time of the animal (mouse or rat) in each zone, comes with 3 USB cables and a webcam.
Using available custom accessories and tools, Bioseb's Thermal Place Preference, 2 Temperatures Choice Nociception Test can be used to run Cold Hot Plate tests, and even Temperature gradient tests. Please contact us
to know more!
Bioseb team presents its respectful thanks to research teams of Prof. Lazdunski (Université de Nice-Sophia Antipolis, France) and Prof. Eschalier (Clermont Université, Clermont-Ferrand, France), who used the Two-Temperatures Choice Test during their recent studies:
The mechano-activated Kþ channels TRAAK and TREK-1 control both warm and cold perception, by J. Noe, K. Zimmermann, J. Busserolles, E. Deval, A. Alloui,S. Diochot, N. Guy, M. Borsotto,P. Reeh, A. Eschalier and M. Lazdunski, EMBO Journal, 2009
(Click here to download this article as a PDF file)