! NEW RESEARCH WORK !
A recent publication
by G Aubertin, M Weiss, F Traversi, D Benameur et al in "International Journal of Obesity" highlights the merits of using Bioseb's Non Invasive Pressure Measurement
: Effects of imidazoline-like drugs on liver and adipose tissues, and their role in preventing obesity and associated cardio-metabolic disorders
The pulse signal level (heart rate) is permanently monitored on the LCD display while an internal pressure transducer is constantly measuring the current pressure applied on the cuff. Measurement process involves several steps which are performed automatically; systolic and diastolic blood pressure and calculated mean blood pressure are visualized in real-time
in the digital display of the control unit. With regards to the systolic pressure, it can be achieved either once the heart pulse disappears or else when the first pulse reappears (user selectable) after the occlusion process.
The system allows a sequential measurement of 6 to 12 animals without having to change pulse transducers and tail cuffs
(Automatic Heater & Scanner option). A built-in air pump ensures automated cuff inflation/deflation at constant and even rate
. In order to avoid deterioration of the animal’s tail after submitting animals to unnecessary high occlusion pressures, the automated deflation is carried out for each animal and separately, once their own systolic value is reached. Real values (without linear regression calculation) are thus achieved while remaining easily traceable
: visualization of the signal through the USB software interface NIBPchart. All units can be indistinctly used for rats, mice or dogs, using specific pulse transducers & cuff.
The requirement of a warm environment for the animals is critical for accurate blood pressure measurements. To provide adequate conditions for NIBP measurement, the system can be use in association to a warming chamber which provides a comfortable and heated environment necessary to produce peripheral vasodilatation and isolate the animal from external noise.
Transducer and cuffs
The N.I.B.P. method in animals is based on the same sphygmomanometric technique used for measuring arterial pressure in humans
. Like its counterpart for human use, this kit is equipped with a cuff to occlude blood flow as well as a transducer to follow pulse evolution throughout the measuring process.
The cuff is inflated until the pulse signal disappears due to the blocking of the caudal artery. Once the signal disappearance has been detected, the cuff deflates automatically. Systolic pressure corresponds then to the pressure value on the cuff when the pulse begins to reappear.
The cuff continues to deflate progressively until the pulse signal recovers its initial value. The pressure reading at this moment corresponds to diastolic pressure. Our system estimates that the pulse has been completely recovered when the signal has reached 80% of the pulse baseline value (and not 80% of the systolic value)
Mean Blood Pressure (MBP)
Once the systolic and diastolic pressure values have been obtained, the system automatically calculates the mean pressure while the cuff is completely deflated for the next evaluation. This value can be calculated using the following formula: MP = DP + 0.33 (SP - DP)
• Systolic blood pressure
• Diastolic blood pressure
• Mean blood pressure
• Heart rate
• Total reliability to realize consecutive measurements on the same animal
• Automated deflation driven separately for each animal (minimize tail lesions)
• Analogic Signal output for recording cuff pressure and pulse signals in a PC
• Automated cuff inflation/deflation at a very constant rate
• The same system can be used for Rat / Dog / Mice
• Real values provided (without any linear regression calculation)
• Easily traceable data (using NIBPchart software)
• Parameters evaluated can be visualized on-line on the digital display
LE56506 Scanner and Heater
Domains of application
• Cardiovascular studies - Non-Invasive Blood Pressure
• Drug screening
• Hypertension/hypotension studies
• Kidney disease studies
• Addison’s disease studies
• Toxin reaction studies
• Heating tunnels (mono or multianimals)
• Card and software for visualising, on PC (through an USB port), the pressure and heart frequency curve. The software allows monitoring, stocking and printing of the curves. It is essential for experiments on mice.
• Rodents restrainers
• Cuffs and detectors