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AUTOMATIC FOOT MISPLACEMENT APPARATUS
(Model: BIO-FMA)
A unique, automatic apparatus for the detailed and objective modeling of walking and walking disorders in mice and/or rats, with respect to motor and psychomotor skills and cognition. An innovative and operator-independent solution for studies on myopathy, ataxia, alcoholism, Parkinson’s and Huntington’s disease, and much more.

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  • IGBMC Strasbourg, France
  • FACULTE DE PHARMACIE Marseille, France
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! NEW RESEARCH WORK ! A recent publication by RD Chavez, P Sohn, R Serra in "PLOS One" highlights the merits of using Bioseb's Automatic Foot misplacement apparatus: Prg4 prevents osteoarthritis induced by dominant-negative interference of TGF-ß signaling in mice

Prg4 prevents osteoarthritis induced by dominant-negative interference of TGF-ß signaling in mice
RD Chavez, P Sohn, R Serra
Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Alabama, United States of America
Published in "PLOS One" (2019-01-10)


Objective Prg4, also known as Lubricin, acts as a joint/boundary lubricant. Prg4 has been used to prevent surgically induced osteoarthritis (OA) in mice. Surgically induced OA serves as a good model for post-traumatic OA but is not ideal for recapitulating age-related OA. Reduced expression of the TGF-_ type II receptor (TGF_R2) is associated with age-related OA in clinical samples, so we previously characterized a mouse model that exhibits OA due to expression of a mutated dominant-negative form of TGF_R2 (DNIIR). Prg4 expression was significantly reduced in DNIIR mice. Furthermore, we showed that Prg4 was a transcriptional target of TGF-

Presentation

'Mouse on a foot misplacement apparutus, by Bioseb
Mouse on the FMA
The Foot Misplacement Apparatus (FMA) is a unique system to objectively detect gait or walking disorders, abnormalities of coordination and motor or reflex problems in small rodents (rat or mouse) in a highly accurate way, by recording the number, position and duration of errors (missteps or paws’ slips as well as tail errors) of an animal walking in an opaque corridor on an horizontal ladder towards a dark compartment.

The ladder bars can be removed to increase the difficulty of the exercise or create traps, and two sets of 77 infrared sensors are placed above and below the ladder to detect the animal’s movement and any paw misplacements, knowing that the number of errors increases with the animals motor deficit and the difficulty of the required task.

The instrument allows you to quantify the recovery of sensorimotor activity, and is of particular interest for studies on myopathy, ataxia, alcoholism, Parkinson’s and Huntington’s disease, recovery from brain or spinal cord injuries, and much more !

Operating principle

2 compartments (a departure and an arrival box) are located on each side of a long horizontal ladder encased in a corridor. The arrival box is a black compartment that attracts the animal, which walks towards the end of the ladder. On each side of the rungs, infra-red beams and sensors ( 77 sets both above and below the ladder) detect the rodent’s movement and are used to recognize and record the exact position and duration errors in motor control (missteps or paw slips between any 2 bars).

Furthermore, the Foot Misplacement system is able to differentiate between fore and hind paws. The space between the rungs can be adjusted to increase the difficulty, and one or more bars can be removed to create traps.

Animals are usually tested across 5 regular runs.

The corridor is removable for an easy and fast cleaning. Rat and mouse corridors are interchangeable to allow you to test small and large rodents (rats or mice) on the same apparatus. One corridor for either rat or mouse is included with the instrument - the other version can be ordered separately.

Key features

• Fully automatic, operator-independent
• Aversive starting box with cold white blinking light
• Interchangeable rat/mouse corridors on the same instrument
• Two sets of 77 infrared sensors provide high accuracy, and great reliability
• Self-powered by USB port from PC
Domains of application

• Functional, toxicological, and genetic models
• Studies on toxicosis, alcoholism
• Resarch on myopathy and ataxia
• Research on Parkinson's and Huntington’s disease
• Recovery from brain or spinal cord injuries

Dedicated Software

Bioseb’s Foot Misplacement Apparatus software records data collected by the sensors and determines parameters for all errors from paws (both front and hind) and tail.

It generates a distinct results file for each animal, which is easily exportable into Excel format. The software also filters sensors information, allows adjustment of the misplacement duration detection, automatically sorts out errors from front legs, back legs and tail and directly calculates the time/report ratio of the course and error rate.

Following recorded parameters are included into the detailed experimental report :
• Total crossing time
• Input and output latency time
• Number of errors (missteps or slips)
• Type of errors (front paws, hind paws or tail)
• Duration of each error
• Position of each error

Screenshots from the foot misplacement system software, by Bioseb Screenshots from the foot misplacement system software, by Bioseb
Screenshots from the foot misplacement system software


Publications (Click on an article to show details and read the abstract)

CENTRAL NERVOUS SYSTEM (CNS)
- Ischemic Strokes -
Therapeutic benefit of a combined strategy using erythropoietin and endothelial progenitor cells after transient focal cerebral ischemia in rats (2013)
Therapeutic benefit of a combined strategy using erythropoietin and endothelial progenitor cells after transient focal cerebral ischemia in rats
L Pellegrini, Y Bennis, B Guillet, L Velly
AIX Merseille UnivINSERM, Merseille, France
Published in "Neurological Research" (2013-11-18)

Objective: Many studies have demonstrated beneficial effects of either erythropoietin (EPO) or endothelial progenitor cell (EPC) treatment in cerebral ischemia. To improve post-ischemic tissue repair, we investigated the effect of systemic administration of endothelial colony-forming cells (ECFCs), considered as relevant endothelial progenitors due to their specific vasculogenic activity, in the presence or absence of EPO, on functional recovery, apoptosis, angiogenesis, and neurogenesis in a transient focal cerebral ischemia model in the adult rat.
Design: Experimental study.
Intervention: The rats were divided into four groups 24 hours after ischemia,, namely control, ECFCs, EPO, and ECFCs+EPO, and received a single intravenous injection of ECFCs (5×106 cells) and/or intraperitoneal administration of EPO (2500 UI/kg per day for 3 days).
Measurement: Infarct volume, functional recovery, apoptosis, angiogenesis, and neurogenesis were assessed at different time points after ischemia.
Main results: The combination of EPO and ECFCs was the only treatment that completely restored neurological function. The ECFCs+EPO treatment was also the most effective to decrease apoptosis and to increase angiogenesis and neurogenesis in the ischemic hemisphere compared to controls and to groups receiving ECFCs or EPO alone.
Conclusion: These results suggest that EPO could act in a synergistic way with ECFCs to potentiate their therapeutic benefits.

MOTOR CONTROL
- Motor Deficits -
Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities (2018)
Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities
M Stewart, PYP Lau, G Banks, RS Bains, E Castroflorio
MRC Harwell Institute, Harwell Campus, Oxfordshire OX11 0RD, UK
Published in "bioRxiv" (2018-08-10)

Loss of function mutations in the human AMPA receptor-associated protein, ferric chelate reductase 1-like (FRRS1L), are associated with a devastating neurological condition incorporating choreoathetosis, cognitive deficits and epileptic encephalopathies. Furthermore, evidence from overexpression and ex vivo studies have implicated FRRS1L in AMPA receptor biogenesis and assembly, suggesting that changes in glutamatergic signalling might underlie the disorder. Here, we investigated the neurological and neurobehavioural correlates of the disorder using a mouse Frrs1l null mutant. The study revealed several neurological defects that mirrored those seen in human patients. We established that mice lacking Frrs1l suffered from a broad spectrum of early-onset motor deficits with no progressive, age-related deterioration. Moreover, Frrs1l-/- mice were hyperactive irrespective of test environment, exhibited working memory deficits and displayed significant sleep fragmentation. Longitudinal electroencephalographic recordings also revealed abnormal EEG in Frrs1l-/- mice. Parallel investigations into disease aetiology identified a specific deficiency in AMPA receptor levels in the brain of Frrs1l-/- mice, while the general levels of several other synaptic components remained unchanged with no obvious alterations in the number of synapses. Furthermore, we established that Frrsl1 deletion results in glycosylation deficits in GLUA2 and GLUA4 AMPA receptor proteins, leading to cytoplasmic retention and a reduction of those specific AMPA receptor levels in the postsynaptic membrane. Overall, this study determines, for the first time in vivo, how loss of FRRS1L function can affect glutamatergic signalling and provides mechanistic insight into the development and progression of a human hyperkinetic disorder.

JOINTS
- Arthritis & Osteoarthritis -
Prg4 prevents osteoarthritis induced by dominant-negative interference of TGF-ß signaling in mice (2019)
Prg4 prevents osteoarthritis induced by dominant-negative interference of TGF-ß signaling in mice
RD Chavez, P Sohn, R Serra
Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Alabama, United States of America
Published in "PLOS One" (2019-01-10)

Objective Prg4, also known as Lubricin, acts as a joint/boundary lubricant. Prg4 has been used to prevent surgically induced osteoarthritis (OA) in mice. Surgically induced OA serves as a good model for post-traumatic OA but is not ideal for recapitulating age-related OA. Reduced expression of the TGF-_ type II receptor (TGF_R2) is associated with age-related OA in clinical samples, so we previously characterized a mouse model that exhibits OA due to expression of a mutated dominant-negative form of TGF_R2 (DNIIR). Prg4 expression was significantly reduced in DNIIR mice. Furthermore, we showed that Prg4 was a transcriptional target of TGF-


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Acquisition speed 10 ms
Number of sensors 154
Dimensions 124 x 28 x 20 (L x W x H cm)
Corridor width 80 mm for rats and 50 mm for mice (1 corridor included)
Space between bars 1/3 cm (customisable)
Aversive starting box using cool white light, adjustable by software
Bar height Adjustable bar height with respect to error sensors
Power supply CE 15-V standard charger
Connector USB compliant

Model:
BIO-FMA
Automatic Foot misplacement apparatus
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