Fully automated instrument for sophisticated detection and analysis of foot faults on the horizontal ladder test for investigation of walking disorders, motor coordination and motor learning. BIOSEB innovated the traditional foot misplacement instrument with synchronized beam break and video detection to assign errors with anatomic precision.
The horizontal ladder test is commonly used to assess the walking capability of mice and rats, and their motor-function in using both front limbs and hind limbs in a coordinated pattern. Applications in neurodegenerative diseases, spinal cord injury, ischemia/stroke models and osteoarthritis are increasingly available in the literature. Until now, an automated scoring test that reports the precise anatomic assignment of error has not been available.
Based on our benchtop Foot-Misplacement Apparatus, the Automatic Ladder Test integrates high speed video detection with existing infrared beam-break detection in an easy-to-use software and a comfortable elevated frame to assign errors with anatomic precision.
An elevated stand supports a laptop, departure box and subject home cage located at opposite ends of a horizontal ladder encased in a corridor. Infra-red beams and sensors (77 sets above and below the ladder) detect the subject’s errors along the length of the ladder while a high speed video camera records the run from below. The included software synchronizes the input and displays the data in real time. The space between the rungs can be adjusted to increase the difficulty, and one or more rungs can be removed to create traps and assess cerebellar learning.
The elevated stand and universal corridor suits both rat and mouse ladder sizes: the width of the alley can be adjusted to the subject size, in order to discourage the subject from changing direction.