The new Forced Swimming Test system from Bioseb uses a dual approach:
Combining a double input from vibrations and video,
the TYC (Train-Your-Computer) algorithm compares both inputs to determine the animal's behavior in real time, making results completely operator-independent.
Experiment manager, analysis module, replay possibilities and the TYC algorithm are some of the features that make FST DUAL SENSOR a very innovative and easy to use instrument for both mice or rats studies (up to 4 animals can be tested at a time), based on the famous Porsolt Test, or "behavioral despair" test
The Forced Swimming Test (FST), also known as Porsolt Swimming Test or 'behavioral despair test', is a common behavioral test for the development and screening of antidepressant and anxiolytic drugs to identify new treatments and understand the biological mechanisms of current treatments.
Bioseb's Forced Swimming Test FST DUAL SENSOR has been designed for rats and mice thanks to the expert work of Dr. Denis DAVID (described in a publication in NEURON in 2009, 'Neurogenesis Dependent and Independent Effects of Fluoxetine in an Animal Model of Anxiety/Depression').
What is the TYC algorithm of Bioseb's new Forced Swimming Test ?
• A new tool that gives the researcher total control when evaluating protocols.
• The researcher is the expert!
• The TYC is taught how to detect the 3 states: Resting/Floating, Swimming, and Climbing/Struggling
The scoring can be learned and then consistently applied with no variability and drift over time! The TYC algorithm is an optimization tool: with a single run manually evaluated by the researcher, one will train the software to use scored parameters, TYC will adjust scoring algorithms to follow the researcher's evaluation rules.
The system allows easy comparison of several approaches, useful in training students, and provides consistency across multiple studies.
Two types of behavior can be observed during the Porsolt Test using FST DUAL SENSOR:
1. Non-depressed rodents (rats or mice), even when unable to escape from the water-filled cylinders, will try to swim and struggle to escape the containers.
2. Depressed rodents will stop trying sooner than non-depressed rodents and start to float in the cylinders, showing behavioural despair.
The floating or immobility time during the forced swimming test is an accurate indication of the effects of anti-depressants and anxiolitics.
• A customized support frame that separates the four beakers, houses the interface and supports the HD camera
• Four graduated beakers for rats or mice, (30 cm diameter for rats, 17 cm diameter for mice; extras available upon request)
• An FST DUAL SENSOR software product with license
• User's manual
- After one-time initial calibration (6 min) : 4% variability
- Data output
- xls type, presented per rat/mouse or per groups & time period (every minutes)
- Dimensions - Animal container
- Graduated Beakers - 17 cm diameter, 20 cm height for mice Graduated Beakers - 30 cm diameter, 50 cm height for rats
- Dimensions - Overall
- 60 x 60 x 120 for the Mouse version 80 x 80 x 180 for the Rat version
- Power supply
- PC USB port
- Sampling rate
- 300Hz, encrypted (glp) averaged and recorded synchronously with video images
- Allows definition of 3 activity levels: Immobility, Swimming and Climbing (struggling)
- Learning machine TYC algorithm : combines video tracking and vibrations Replay and control : At any time, remotely. Scoring tool included for calibration, may be used for training purpose and comparison with expert