Activity, Motor Control & Coordination
Pain - Thermal Allodynia / Hyperalgesia
Pain - Spontaneous Pain - Postural Deficit
Pain - Mechanical Allodynia / Hyperalgesia
Anxiety & Depression Disorder
Learning/Memory - Attention - Addiction
Physiology & Respiratory Research
Metabolism - Food & Drink Intake
Pain
Metabolism
Motor control
Neurodegeneration
Cross-disciplinary subjects
Muscular system
General activity
Mood Disorders
Other disorders
Joints
Central Nervous System (CNS)
Sensory system
Bioseb on booth #14 at OARSI 2024 in Vienna Authors
Latremoliere A et al
Lab
Kirby Neurobiology Center, Boston ChildrenÕs Hospital, Boston, MA, USA
Journal
Cell Reports
Abstract
We generated a knockout mouse for the neuronal-specific _-tubulin isoform Tubb3 to investigate its role in nervous system formation and maintenance. Tubb3_/_ mice have no detectable neurobehavioral or neuropathological deficits, and upregulation of mRNA and protein of the remaining _-tubulin isotypes results in equivalent total _-tubulin levels in Tubb3_/_ and wild-type mice. Despite similar levels of total _-tubulin, adult dorsal root ganglia lacking TUBB3 have decreased growth cone microtubule dynamics and a decreased neurite outgrowth rate of 22% inÊvitro and inÊvivo. The effect of the 22% slower growth rate is exacerbated for sensory recovery, where fibers must reinnervate the full volume of the skin to recover touch function. Overall, these data reveal that, while TUBB3 is not required for formation of the nervous system, it has a specific role in the rate of peripheral axon regeneration that cannot be replaced by other _-tubulins.
BIOSEB Instruments Used:
Cold Hot Plate Test (BIO-CHP)
