BMSCs_derived Exosomes ameliorate pain via abrogation of aberrant nerve invasion in subchondral bone in lumbar facet joint osteoarthritis

Authors
J Li, Z Ding, Y Li, W Wang, J Wang, H Yu, A Liu, J Miao et al

Lab
Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, 410008 China

Journal
Journal of Orthopaedic Research

Abstract
Lumbar facet joint osteoarthritis (LFJ OA) is regarded as one of the common causes of low back pain (LBP). The pathogenesis and underlying mechanism of this disease are largely unknown, there is still no effective disease_modifying therapy. This study aims to investigate the efficacy of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on the pathogenesis and behavioral signs of LBP in the LFJ OA mouse model. The pathogenetic change in cartilage and aberrant nerve invasion in the subchondral bone of LFJ in a mouse model after treatment with BMSC_exosomes was evaluated. BMSC_exosomes could relieve pain via abrogation of aberrant CGRP_positive nerve and abnormal H_type vessel formation in the subchondral bone of LFJ. Moreover, BMSC_exosomes attenuated cartilage degeneration and inhibited tartrate_resistant acid phosphatase expression and RANKL_RANK_TRAF6 signaling activation to facilitate subchondral bone remodeling. These results indicated that BMSC_exosomes could relive behavioral signs of LBP and pathological processes in LFJ OA. BMSC_exosomes have a prominent protective effect and might be a potential therapeutic option for the treatment of LFJ OA causing LBP.

BIOSEB Instruments Used:
SMALGO: SMall animal ALGOmeter (BIO-SMALGO)