Activated astrocytes enhance the dopaminergic differentiation of stem cells and promote brain repair through ?bFGF
Envoyer une demande de publication
close

Demande de publication

Merci pour votre intérêt pour nos produits et votre demande concernant cette publication, qui vous sera envoyée si le chercheur et le journal l'autorisent. Notre équipe commerciale vous contactera rapidement.



Authors
Fan Yang, Yunhui Liu, Jie Tu, Jun Wan, Jie Zhang, Bifeng Wu, Shanping Chen, Jiawei Zhou, Yangling Mu & Liping Wang


Lab
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China

Journal
Nature Communications

Abstract
Astrocytes provide neuroprotective effects against degeneration of dopaminergic (DA) neurons and play a fundamental role in DA differentiation of neural stem cells. Here we show that light illumination of astrocytes expressing engineered channelrhodopsin variant (ChETA) can remarkably enhance the release of ?basic fibroblast growth factor (?bFGF) and significantly promote the DA differentiation of human embryonic stem cells (hESCs) in vitro. Light activation of transplanted astrocytes in the substantia nigra (SN) also upregulates ?bFGF levels in vivo and promotes the regenerative effects of co-transplanted stem cells. Importantly, upregulation of ?bFGF levels, by specific light activation of endogenous astrocytes in the SN, enhances the DA differentiation of transplanted stem cells and promotes brain repair in a mouse model of Parkinson’s disease (PD). Our study indicates that astrocyte-derived ?bFGF is required for regulation of DA differentiation of the stem cells and may provide a strategy targeting astrocytes for treatment of PD.

BIOSEB Instruments Used:
Grip strength test (BIO-GS3)

Produits associés

Partager ce contenu