The transcription factor _FosB is recruited by inflammatory pain-
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Authors
O. E. Luis-Delgado, M. Barrot, J.-L. Rodeau, P. G. Ulery, M.-J. Freund-Mercier et al.


Lab
Institut des Neurosciences Cellulaires et Intégratives, CNRS/Université Louis Pasteur, Strasbourg, Nociception and Pain department, France ; The University of Texas Southwestern Medical Center, Dallas, USA.

Journal
Journal of Neurochemistry

Abstract
_FosB, a stable splice variant of FosB, has been proposed to mediate persistent brain adaptation in response to several chronic perturbations, but it has not yet been considered in the context of sustained pain. Inflammatory pain induces neuronal plasticity that can result in persistent alteration of nociceptive pathways. This neuronal plasticity can partly result from changes in gene expression controlled by transcription factors. In the present study, we analyse the capacity of carrageenan-mediated inflammation to induce _FosB in the spinal cord. We found that hind-paw inflammation increases FosB-like immunoreactivity in the superficial layers of rat lumbar spinal cord for at least 7 days. This induction parallels mechanical hyperalgesia and is maximal in the dorsal horn of segment L4 of the spinal cord which corresponds to the primary nociceptive afferent regions from the hind paw. We identified this FosB-like signal as _FosB by comparing data obtained with antibodies raised against either an epitope present in both FosB and _FosB, or the FosB C-terminal region that is deleted in _FosB. The week-lasting changes in _FosB highlight the interest in this protein as a molecular marker of sustained pain, and suggest a role of this transcription factor in pain-related plasticity within the spinal cord.

BIOSEB Instruments Used:
Rodent pincher - analgesia meter (BIO-RP-M)

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