Lifespan extension with preservation of hippocampal function in aged system xc_-deficient male mice

Authors
L Verbruggen, G Ates, O Lara et al

Lab
Laboratory of Neuro-Aging & Viro-Immunotherapy, Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Brussels, Belgium

Journal
Molecular Psychiatry

Abstract
The cystine/glutamate antiporter system xc_ has been identified as the major source of extracellular glutamate in several brain regions as well as a modulator of neuroinflammation, and genetic deletion of its specific subunit xCT (xCT_/_) is protective in mouse models for age-related neurological disorders. However, the previously observed oxidative shift in the plasma cystine/cysteine ratio of adult xCT_/_ mice led to the hypothesis that system xc_ deletion would negatively affect life- and healthspan. Still, till now the role of system xc_ in physiological aging remains unexplored. We therefore studied the effect of xCT deletion on the aging process of mice, with a particular focus on the immune system, hippocampal function, and cognitive aging. We observed that male xCT_/_ mice have an extended lifespan, despite an even more increased plasma cystine/cysteine ratio in aged compared to adult mice. This oxidative shift does not negatively impact the general health status of the mice. On the contrary, the age-related priming of the innate immune system, that manifested as increased LPS-induced cytokine levels and hypothermia in xCT+/+ mice, was attenuated in xCT_/_ mice. While this was associated with only a very moderate shift towards a more anti-inflammatory state of the aged hippocampus, we observed changes in the hippocampal metabolome that were associated with a preserved hippocampal function and the retention of hippocampus-dependent memory in male aged xCT_/_ mice. Targeting system xc_ is thus not only a promising strategy to prevent cognitive decline, but also to promote healthy aging.

BIOSEB Instruments Used:
Grip strength test (BIO-GS3)