Metformin induces muscle atrophy by transcriptional regulation of myostatin via HDAC6 and FoxO3a

Authors
MJ Kang, JW Moon, JO Lee et al

Lab
Department of Anatomy, Korea University College of Medicine, Seoul, Republic of Korea

Journal
Journal of Cachexia, Sarcopenia and Muscle

Abstract
Myostatin expression was investigated at the protein and transcript levels after metformin administration. To investigate the pathways associated with myostatin signalling, we used real-time polymerase chain reaction, immunoblotting, luciferase assay, chromatin immunoprecipitation assay, co-immunoprecipitation, immunofluorescence, primary culture, and confocal microscopy. Serum analysis, physical performance, and immunohistochemistry were performed using our in vivo model.

BIOSEB Instruments Used:
Grip strength test (BIO-GS3)